Expression of the Dominant-Negative Regulator Id4 Is Induced during Adipocyte Differentiation
| Autor: | Laura K. Sanders, Heping Chen, Barbara A. Christy, Gina C. Schatteman, Robert J. Christy, Yi Chinn Weng |
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| Rok vydání: | 1999 |
| Předmět: |
Time Factors
Biophysics Dominant negative Regulator Down-Regulation Adipose tissue Biology Biochemistry Dexamethasone Mice chemistry.chemical_compound 1-Methyl-3-isobutylxanthine Adipocyte Adipocytes Transcriptional regulation Animals Humans Insulin RNA Messenger Cycloheximide Molecular Biology Gene Messenger RNA Helix-Loop-Helix Motifs Proteins Cell Differentiation 3T3 Cells Cell Biology Molecular biology Up-Regulation DNA-Binding Proteins chemistry Inhibitor of Differentiation Proteins Transcription Factors Hormone |
| Zdroj: | Biochemical and Biophysical Research Communications. 256:614-619 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1999.0386 |
| Popis: | Expression of the Id4 gene was investigated during differentiation of 3T3-L1 preadipocytes into mature adipocytes. Id4 is a member of a family encoding non-DNA binding helix-loop-helix proteins proposed to inhibit the activity of basic HLH (bHLH) proteins important in many developmental processes. We show here that Id4 expression is low in confluent preadipocytes and rapidly induced by treatment with the combination of hormones which causes differentiation into mature adipocytes. Id4 expression is also induced by treatment with individual hormones, especially dexamathasone. Id4 mRNA can be detected in mouse and human adipose tissue. Genes encoding E-proteins (bHLH proteins known to interact with and be regulated by Id proteins) are expressed and regulated during differentiation in 3T3-L1 cells. These data suggest that the Id4 transcriptional regulator is playing a role in adipose cell differentiation and suggest that DNA-binding HLH proteins may also be important in regulation of differentiation of these cells. |
| Databáze: | OpenAIRE |
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