Effects of non-ionic surfactants on the interactions between cellulases and tannic acid: a model system for cellulase-poly-phenol interactions
| Autor: | Kim Borch, M.D. Sweeny, Søren Nymand Olsen, K.C. McFarland, Leigh Murphy, Peter Westh, Christina Bohlin |
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| Rok vydání: | 2011 |
| Předmět: |
Non ionic
Octoxynol Bioengineering Cellulase Applied Microbiology and Biotechnology Biochemistry Lignin Models Biological chemistry.chemical_compound Surface-Active Agents Adsorption Tannic acid Organic chemistry Phenol Cellulases health care economics and organizations chemistry.chemical_classification biology Calorimetry Differential Scanning Hydrolysis technology industry and agriculture Isothermal titration calorimetry Enzyme chemistry biology.protein Tannins Biotechnology |
| Zdroj: | Enzyme and microbial technology. 49(4) |
| ISSN: | 1879-0909 |
| Popis: | Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS-lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In the current work we address this hypothesis using tannic acid (TAN) as a general poly-phenolic model compound (for lignin and soluble phenolics) and measure the mutual interactions of cellulases (CBHI, CBHII, EGI, EGII and BG), TAN and NIS (Triton X-100) using isothermal titration calorimetry (ITC). The experimental results suggest rather strong enzyme-specific interactions with TAN in reasonable agreement with enzyme specific lignin inhibition found in the literature. Enzyme-TAN interactions were disrupted by the presence of NIS by a mechanism of strong TAN-NIS interaction. The presence of NIS also alleviated the inhibitory effect of TAN on cellulase activity. All together the current work provides strong indications that favorable NIS-poly-phenol interactions alleviate non-productive cellulase-poly-phenol interactions and hence may provide a mechanism for the accelerating effect of NIS on lignocellulose hydrolysis. |
| Databáze: | OpenAIRE |
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