Capacitative calcium influx in human epithelial breast cancer and non-tumorigenic cells occurs through Ca2+ entry pathways with different permeabilities to divalent cations
| Autor: | Ricardo Boland, Guillermo Vazquez, Carolina Baldi |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
inorganic chemicals
Cell Membrane Permeability Cations Divalent Cell Kinetics Breast Neoplasms Biochemistry Ion Channels Cell Line Divalent Breast cancer Tumor Cells Cultured medicine Humans Enzyme Inhibitors Molecular Biology chemistry.chemical_classification Cancer Epithelial Cells Cell Biology medicine.disease Cell biology medicine.anatomical_structure chemistry SKBR3 Cell culture Carcinogens Thapsigargin Calcium Homeostasis |
| Zdroj: | Journal of Cellular Biochemistry. 88:1265-1272 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.10471 |
| Popis: | The operation of capacitative Ca2+ entry (CCE) in human breast cancer (SKBR3) and non-tumorigenic (HBL100) cell lines was investigated as an alternative Ca2+ entry route in these cells. Ca2+ readdition after thapsigargin-induced store depletion showed activation of CCE in both cell lines. SKBR3 cells exhibited retarded store depletion and CCE decay kinetics compared to the non-tumorigenic HBL100 cells, suggesting alterations in Ca2+ homeostasis. CCE was also highly permeable to Mn2+ and to a lesser extent to Sr2+, but not to Ba2+. In HBL100 cells, CCE is contributed (30%) by a Ca2+/Mn2+ permeable route insensitive to low (1 μM) Gd3+ and a Ca2+/Sr2+/Mn2+ permeable non-selective pathway (70%) sensitive to 1 μM Gd3+. In SKBR3 cells, the relative contribution to CCE of both routes was opposite to that in non-tumorigenic cells. J. Cell. Biochem. 88: 1265–1272, 2003. © 2003 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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