The influence of intrinsic coagulation pathway on blood platelets activation by oxidized cellulose
| Autor: | Jiří Suttnar, M Pecka, P Krízová, P Salaj, Jiří Homola, L Másová, Jan E. Dyr |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Serotonin
Platelet Aggregation Oxidized cellulose Biomedical Engineering chemistry.chemical_element Biocompatible Materials Coagulation Protein Disorders In Vitro Techniques Calcium Fibrinogen Hemostatics Biomaterials chemistry.chemical_compound Materials Testing medicine Humans Cellulose Oxidized Platelet Platelet activation Cellulose Blood Coagulation Whole blood Metals and Alloys Platelet Activation Coagulation Biochemistry chemistry Ceramics and Composites medicine.drug |
| Zdroj: | Journal of Biomedical Materials Research Part A. :274-280 |
| ISSN: | 1552-4965 1549-3296 |
| DOI: | 10.1002/jbm.a.31060 |
| Popis: | Oxidized cellulose is an effective hemostat that works naturally to aid in blood coagulation. The mechanism of its action is not very well understood. Little effect on blood coagulation, but a pronounce decrease in platelet count has been reported upon the addition of the oxidized cellulose to the whole blood. As a marker of platelet activation and aggregation we used serotonin release reaction and turbidity changes in time. We found that oxidized cellulose did not activate washed platelets reconstituted in plasma-free medium or plasma-free medium with fibrinogen; no reduction of platelet count was observed. Serotonin release in platelet-rich plasma incubated with oxidized cellulose started in the range from 5 to 10 min. Serotonin release from platelets reconstituted in plasma deficient in either coagulation factor V, VIII, IX, or XII was delayed. Blood platelets activation by oxidized cellulose requires calcium ions present in dispersion of oxidized cellulose. Factor XIII deficiency had no influence on blood platelets activation by oxidized cellulose. Our results clearly indicate the significance of intrinsic coagulation pathway activation on blood platelets activation by oxidized cellulose and so indirectly on the hemostyptic effect of oxidized cellulose. |
| Databáze: | OpenAIRE |
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