CRMP2 mediates Sema3F-dependent axon pruning and dendritic spine remodeling
| Autor: | Martin Balastik, Eran Perlson, Romana Weissova, Roy Maimon, Thomas Misgeld, Tomas Petrasek, Marie Kleisnerova, Radislav Sedlacek, Jakub Ziak, Barbora Pukajova, Monika S. Brill, Mengzhe Wang, Xunlei Zhou, Ales Stuchlik, Gonzalo Alvarez-Bolado, Petr Kasparek, Kateřina Jeřábková, Martina Janikova |
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| Rok vydání: | 2019 |
| Předmět: |
Dendritic spine
Autism Spectrum Disorder Dendritic Spines Nerve Tissue Proteins Semaphorins Biology Biochemistry Synapse Mice 03 medical and health sciences 0302 clinical medicine Semaphorin ddc:570 Genetics medicine Biological neural network Animals Axon Molecular Biology genetics [Nerve Tissue Proteins] 030304 developmental biology Mice Knockout Neurons 0303 health sciences physiology [Membrane Proteins] Neuronal Plasticity genetics [Intercellular Signaling Peptides and Proteins] Membrane Proteins SEMA3A Articles medicine.anatomical_structure nervous system Intercellular Signaling Peptides and Proteins physiology [Nerve Tissue Proteins] Axon guidance Neuron Collapsin response mediator protein family Neuroscience 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | EMBO reports 21(3), e48512 (2020). doi:10.15252/embr.201948512 EMBO Rep |
| Popis: | Regulation of axon guidance and pruning of inappropriate synapses by class 3 semaphorins is key to development of neural circuits. Collapsin response mediator protein 2 (CRMP2) has been shown to regulate axon guidance by mediating Semaphorin 3A (Sema3A) signaling and its dysfunction has been linked to schizophrenia, however, nothing is known about its role in the synapse pruning. Here, using newly generated crmp2−/− mice we demonstrate that while CRMP2 has only a moderate effect on Sema3A-dependent axon guidance in vivo, it is essential for Sema3F-dependent axon pruning and dendritic spine remodeling. We first demonstrate that CRMP2 deficiency interferes with Sema3A signaling in compartmentalized neuron cultures and leads to a mild defect in axon guidance in peripheral nerves and corpus callosum. Strikingly, we show that crmp2−/− mice display more prominent defects in dendritic spine pruning and stereotyped axon pruning in hippocampus and visual cortex consistent with impaired Sema3F signaling and with autism spectrum disorder (ASD)-rather than schizophrenia-like phenotype. Indeed, we demonstrate that CRMP2 mediates Sema3F-induced axon retraction in primary neurons and that crmp2−/− mice display early postnatal behavioral changes linked to ASD. In conclusion, we demonstrate that CRMP2 is an essential mediator of Sema3F-dependent synapse pruning and its dysfunction in early postnatal stages shares histological and behavioral features of ASD. |
| Databáze: | OpenAIRE |
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