Linking genotoxicity and cytotoxicity with membrane fluidity: A comparative study in ovarian cancer cell lines following exposure to auranofin
Autor: | Dennis Yiannakis, Nicholas J.F. Dodd, Awadhesh N. Jha, Deepu Oommen, Rana Moyeed |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Auranofin Membrane Fluidity DNA repair Health Toxicology and Mutagenesis Apoptosis Biology 03 medical and health sciences 0302 clinical medicine Tumor Cells Cultured Genetics Membrane fluidity medicine Humans Cytotoxicity Ovarian Neoplasms Mutagenicity Tests medicine.disease 030104 developmental biology Cell culture Antirheumatic Agents 030220 oncology & carcinogenesis Cancer cell Immunology Cancer research Female Reactive Oxygen Species Ovarian cancer DNA Damage medicine.drug |
Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 809:43-49 |
ISSN: | 1383-5718 |
Popis: | Auranofin, an organogold compound classified as an anti-rheumatic agent is under phase 2 clinical trials for re-purposing to treat recurrent epithelial ovarian cancer. We have reported earlier that Breast cancer 1, early onset (BRCA1) mutant ovarian cancer cells exhibit increased sensitivity to auranofin. BRCA1 is a DNA repair protein whose functional status is critical in the prognosis of ovarian cancer. Apart from DNA repair capability of cancer cells, membrane fluidity is also implicated in modulating resistance to chemotherapeutics. We report here that membrane fluidity influences the sensitivity of ovarian cancer cell lines, OVCAR5 and IGROV1, to auranofin. Electron spin resonance (ESR) analysis revealed a more fluidized membrane in IGROV1 compared to OVCAR5. Interestingly, IGROV1 cells were more sensitive to auranofin induced cytotoxicity than OVCAR5. In comparison to OVCAR5, IGROV1 cells also exhibited an increased number of DNA double strand breaks (DSBs) upon auranofin treatment as assessed by 53BP1 immunostaining. Furthermore, correlation analysis demonstrated a strong positive correlation (r=0.856) between membrane fluidity and auranofin sensitivity in these cell lines. Auranofin-treated IGROV1 cells also exhibited increased cellular oxidation and apoptosis. Anti-oxidant, N-acetyl cysteine (NAC) inhibited the cellular oxidation and apoptosis in auranofin-treated ovarian cancer cells suggesting reactive oxygen species (ROS) mediate the anti-cancer properties of auranofin. Overall, our study suggests that auranofin mediates its cytotoxicity via ROS production in ovarian cancer cells which correlates positively with membrane fluidity. |
Databáze: | OpenAIRE |
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