Evaluation of potential biomarkers for the diagnosis and monitoring of Systemic Lupus Erythematosus using the Cytometric Beads Array (CBA)
| Autor: | Luan Carlos Vieira Alves, Walter Batista Cicarini, L.F. Ferreira, Maria da Gloria Carvalho, D.C. Calderaro, Edna Afonso Reis, Vicente de Paulo Coelho Peixoto de Toledo, Fernanda F. C. Nunes, Joana Starling De Carvalho, Isabela Moreira Gondim, Paulo Madureira de Pádua, Tânia Mara Pinto Dabés Guimarães, Gilda Aparecida Ferreira |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Clinical Biochemistry Disease Biochemistry 03 medical and health sciences 0302 clinical medicine Internal medicine Healthy volunteers High activity Medicine Humans Lupus Erythematosus Systemic skin and connective tissue diseases business.industry Biochemistry (medical) Low activity General Medicine Middle Aged Serum samples Flow Cytometry Inflammatory biomarkers Rheumatology 030104 developmental biology 030220 oncology & carcinogenesis Potential biomarkers Immunology Cytokines Female business Biomarkers |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 499 |
| ISSN: | 1873-3492 |
| Popis: | Systemic Lupus Erythematosus (SLE) is an autoimmune, multisystemic disease. Currently diagnosis depends on complex criteria developed by the American College of Rheumatology. Moreover, the lack of specific biomarkers also challenges the diagnosis.Inflammatory biomarkers such as IL-8, IP-10, MIG, MIP-1α and RANTES were measured in serum samples from SLE patients and subjects in control groups (patients with other autoimmune diseases and healthy individuals). Forty-six SLE patients (22 patients with low activity, SLEDAI-2 K ≤ 4, 24 patients with moderate/high activity, SLEDAI-2 K 4), 42 patients with other autoimmune diseases (OAD group), and 8 healthy volunteers participated in this study.MIG (p .001) and RANTES (p .001) concentrations in SLE patients and healthy controls, and IP-10 concentrations in SLE patients with different disease activities (low activity, p .01, moderate/high activity, p .05) differed significantly. IL-8 (p .001) and MIP-1α (p .001) concentrations in SLE patients differed from those in patients from the OAD group. IL-8 (p .05), IP-10 (p .01), MIG (p .05), MIP-1α (p .001), and RANTES (p .05) were correlated with SLE activity; their concentrations in SLE patients with low and moderate/high activity differed significantly.Given the findings of this study, one can envision the possibility of future use of some of these cytokines to assist in the screening of SLE patients, or even in monitoring disease activity. |
| Databáze: | OpenAIRE |
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