Multiparametric Highly Sensitive Chemiluminescence Immunoassay for Quantification of β-Lactam-Specific Immunoglobulin E
| Autor: | Pedro Quintero-Campos, María José Juárez, Ángel Maquieira, Sergi Morais |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Luminescence
medicine.drug_class Antibiotics Drug allergy 010402 general chemistry Immunoglobulin E beta-Lactams 01 natural sciences Analytical Chemistry Antibody Specificity Limit of Detection QUIMICA ANALITICA medicine Humans Immunoassay biology medicine.diagnostic_test Chemistry QUIMICA INORGANICA 010401 analytical chemistry Amoxicillin medicine.disease In vitro 0104 chemical sciences 3. Good health Penicillin Immunology biology.protein medicine.drug Piperacillin |
| Zdroj: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| ISSN: | 1520-6882 |
| Popis: | [EN] beta-lactams (BLCs) are the most widely used antibiotics and consequently the most common cause of drug allergy in the world. The diagnosis of drug allergy is complex and represents a serious challenge that includes a wide variety of methods. In vitro tests are based on the immunological determination of allergen-specific IgE, but the tests in the market lack the required sensitivity and specificity. In addition, the large sample volume, long incubation times, and single-plex configuration have brought their use into question to complement the clinical information. Here, we report a chemiluminescence immunoassay (CLIA) for multiparametric quantification of specific IgE to penicillin G, penicillin V, amoxicillin, and piperacillin, using histone H1 as a carrier. The developed CLIA allowed the determination of BLC-specific IgE below 0.1 IU/mL, thus allowing identification of allergic patients with better sensitivity, using only 25 mu L of a sample (serum). The immunoassay was successfully applied in a cohort of 140 human serum samples, showing good sensitivity (64.6%) as well as specificity (100%), which significantly improve the predictive character of existing BLC-allergy in vitro tests. P.Q.C. acknowledges financial support from Generalitat Valenciana through the research staff-training program (GVA ACIF/2018/173). This research has been funded by the H2020 Program (project COBIOPHAD, grant agreement No. 688448), which is an initiative of the Photonics PPP (www.photonics21.org). |
| Databáze: | OpenAIRE |
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