Studies on the sequence of the 3'-terminal region of turnip-yellow-mosaic-virus RNA

Autor:	Anne-Lise Haenni, Uttam L. RajBhandary, M. Silberklang, Alain Prochiantz
Rok vydání:	1977
Předmět:	Nuclease Turnip yellow mosaic virus Oligoribonucleotides biology Base Sequence RNase P Oligonucleotide Nucleic acid sequence RNA biology.organism_classification Biochemistry Molecular biology Virus Plant Viruses Ribonucleases Mosaic Viruses biology.protein RNA Viral Ribonuclease T1 Gene
Zdroj:	European journal of biochemistry. 72(3)
ISSN:	0014-2956
Popis:	A fragment representing the 3′-terminal ‘tRNA-like’ region of turnip yellow mosaic (TYM) virus RNA has been purified following incubation of intact TYM virus RNA with Escherichia coli‘RNase P’. This fragment, which is 112 ± 3-nucleotides long has been completely digested with T1 RNase and pancreatic RNase and all the oligonucleotides present in such digests have been sequenced using 32P-end labelling techniques in vitro. The TYM virus RNA fragment is free of modified nucleosides and does not contain a G-U-U-C-R sequence. Using nuclease P1 from Penicillium citrinum, the sequence of 26 nucleotides from the 5′ end and 16 nucleotides from the 3′ end of this fragment has been deduced. The nucleotide sequence at the 5′ end of the TYM virus RNA fragment indicates that this fragment includes the end of the TYM virus coat protein gene.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a55bc8ef220fc217752c14717a087c8a https://pubmed.ncbi.nlm.nih.gov/402264 Zobrazit plný text záznamu Plný text ve formátu PDF