Association of HLA-G+3142 C > G polymorphism and breast cancer in Tunisian population

Autor: Hanene Chelbi, Ahmed Baligh Laaribi, Refaat Sebai, Amel Mezlini, Hamza Ben Yahia, Amna Ben Hassine, Olfa Dziri, Nour Zidi, Wafa Babay, Nadia Boujelebene, Hela Rifi, Inès Zidi
Přispěvatelé: Université de Tunis El Manar (UTM), Departement Medecine Oncologique [Tunis], Institut Salah Azaiz [Tunis] (ISA), Departement Anatomopathologie [ISA, Tunis], Laboratoire de Parasitologie Médicale, Biotechnologies et Biomolécules (LR11IPT06), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis (FST)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Carcinogenesis
MICRORNAS
[SDV]Life Sciences [q-bio]
VARIANTS
SUSCEPTIBILITY
medicine.disease_cause
0302 clinical medicine
Breast cancer
Gene Frequency
HLA-G
Genotype
ANTIGEN-G EXPRESSION
MESH: Breast Neoplasms/pathology
Sequence Deletion
MESH: Genetic Association Studies
MESH: Genotype
Genetics
MESH: Aged
MESH: Middle Aged
MESH: Risk
PLASMA
Age Factors
MESH: Genetic Predisposition to Disease
MESH: Neoplasm Staging
Middle Aged
MESH: Carcinogenesis
+3142 C > G
3. Good health
MESH: Breast Neoplasms/genetics
SHLA-G MOLECULES
030220 oncology & carcinogenesis
+3142 C>
SECRETION
Female
MESH: Tunisia
Adult
Risk
Tunisia
HLA-G GENE
CARCINOMA
Immunology
Breast Neoplasms
Human leukocyte antigen
Biology
REGION
03 medical and health sciences
MESH: Polymorphism
Genetic
medicine
Carcinoma
MESH: Gene Frequency
Humans
Genetic Predisposition to Disease
Allele
Polymorphism
MESH: HLA-G Antigens/genetics
Allele frequency
Genetic Association Studies
Aged
Neoplasm Staging
HLA-G Antigens
MESH: Age Factors
14-bp Insertion/deletion
Polymorphism
Genetic
MESH: Humans
MESH: Sequence Deletion/genetics
MESH: Adult
medicine.disease
Molecular biology
MESH: Female
030215 immunology
Zdroj: Immunologic Research
Immunologic Research, Humana Press, 2016, ⟨10.1007/s12026-015-8782-6⟩
Immunologic Research, Humana Press, 2016, 64 (4), pp.961-968. ⟨10.1007/s12026-015-8782-6⟩
ISSN: 0257-277X
DOI: 10.1007/s12026-015-8782-6⟩
Popis: International audience; HLA-G is highly expressed in cancer. Also, it is associated to its progression. Here, we explored the relationship between two HLA-G polymorphisms with breast cancer (BC) and tried to make a correlation with sHLA-G levels. We genotyped 104 patients with BC and 83 controls (CTRL) for HLA-G 14-bp insertion/deletion (Ins/Del) and HLA-G +3142 C > G polymorphisms. The mutations were identified with PCR and PCR-RFLP. The sHLA-G dosage was performed on plasma samples by a specific ELISA. A significant association with BC was found concerning the G allele in the +3142 C > G polymorphism (p = 0.0004). The G/G genotype is the protective genotype (1 % in BC patients vs. 13.1 % in CTRL, OR 0.065, 95 % CI 0.008-0.523). No statistically significant differences were observed for the 14-bp Ins/Del polymorphism between BC patients and controls frequencies. The protection by G/G genotype of +3142 C > G polymorphism is maintained in young patients (< 50 years, p = 0.0006) and in early-diagnosed BC patients (< 50 years, p = 0.0033). In addition, an association was found between the haplotypes inferred by both HLA-G polymorphisms and BC susceptibility. Indeed, the (DelG) haplotype is found as the protective haplotype against BC (OR 0.269, 95 % CI 0.081-0.895, p = 0.023). The ELISA dosage of sHLA-G revealed increased levels in BC compared to CTRL (p < 0.0001). We demonstrated also that sHLA-G is closely associated with advanced stages of BC without significance. sHLA-G is increased in TNM IV and SBR III subgroups. It is also enhanced in patients with a tumor size over 20 mm and in triple-negative patients. Taken together, our findings demonstrate, for the first time, the association of HLA-G +3142 C > G polymorphism with BC susceptibility in Tunisian population. Our results revealed also a potential implication of sHLA-G in advanced stages of BC.
Databáze: OpenAIRE
Externí odkaz: