High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry (HPLC-ESI-MSn) Analysis and Bioactivity Useful for Prevention of 'Diabesity' of Allium commutatum Guss
| Autor: | Vincenzo Ilardi, Stefania Sut, Stefano Dall'Acqua, Mariarosaria Leporini, Monica Rosa Loizzo, Maurizio Bruno, Tiziana Falco, Rosa Tundis, Vincenzo Sicari |
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| Přispěvatelé: | Loizzo M.R., Tundis R., Sut S., Dall'Acqua S., Ilardi V., Leporini M., Falco T., Sicari V., Bruno M. |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
DPPH Allium commutatum Electrospray ionization Bulbs Alliin High-performance liquid chromatography Antioxidants Allium 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Antioxidant activity Tandem Mass Spectrometry Carbohydrate-hydrolysing enzymes inhibition Enzyme Inhibitors Garlic Chromatography High Pressure Liquid 030109 nutrition & dietetics ABTS Chromatography biology Plant Extracts 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Lipase inhibition Italy chemistry Chemistry (miscellaneous) Aerial parts Quercetin Food Science |
| Zdroj: | Plant Foods for Human Nutrition. 75:124-130 |
| ISSN: | 1573-9104 0921-9668 |
| DOI: | 10.1007/s11130-019-00782-2 |
| Popis: | This study aimed at evaluating and comparing the chemical profile obtained by HPLC-ESI-MSn analysis, the inhibitory activity of enzymes linked to obesity (α-amylase, α-glucosidase, and lipase) and the antioxidant properties (DPPH, ABTS, FRAP, and β-carotene bleaching tests) of ethanol extracts of bulbs (BE) and aerial parts (APE) from Allium commutatum Guss. (known in Italy as “aglio delle isole”). The chemical profile revealed alliin as the main abundant compound with values of 31.5 and 38.8mg/g extract for BE and APE, respectively. APE is rich also in quercetin (38.5mg/g extract) and luteolin (31.8mg/g extract). Bulbs extract exhibited the highest activity as inhibitor of enzymes linked to obesity. Except for DPPH test, APE showed the highest antioxidant potential with IC50 of 7.6 and 56.6μg/mL in ABTS and β-carotene bleaching test after 60min of incubation, respectively. In conclusion, the present investigation revealed A. commutatum bulbs and aerial parts as a promising source of inhibitors of enzyme linked to the obesity and of antioxidant compounds. |
| Databáze: | OpenAIRE |
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