Tissue factor-factor VIIa–specific up-regulation of IL-8 expression in MDA-MB-231 cells is mediated by PAR-2 and results in increased cell migration
| Autor: | Brit B. Sørensen, Usha R. Pendurthi, Gertrud M. Hjortoe, Lars Christian Petersen, Samir K. Mandal, Peder Lisby Nørby, Tatjana Albrektsen, L. Vijaya Mohan Rao |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Angiogenesis
medicine.medical_treatment Immunology Breast Neoplasms Factor VIIa Biology Biochemistry Article Thromboplastin Tissue factor chemistry.chemical_compound Thrombin Cell Movement Cell Line Tumor medicine Humans Receptor PAR-2 Neoplasm Invasiveness RNA Messenger RNA Neoplasm Interleukin 8 Factor VII Interleukin-8 Cell migration Cell Biology Hematology Up-Regulation Cell biology Cytokine chemistry Cell culture Female medicine.drug |
| Zdroj: | Blood. 103:3029-3037 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2003-10-3417 |
| Popis: | Tissue factor (TF), the cellular receptor for factor VIIa (FVIIa), besides initiating blood coagulation, is believed to play an important role in tissue repair, inflammation, angiogenesis, and tumor metastasis. Like TF, the chemokine interleukin-8 (IL-8) is shown to play a critical role in these processes. To elucidate the potential mechanisms by which TF contributes to tumor invasion and metastasis, we investigated the effect of FVIIa on IL-8 expression and cell migration in a breast carcinoma cell line, MDA-MB-231, a cell line that constitutively expresses abundant TF. Expression of IL-8 mRNA in MDA-MB-231 cells was markedly up-regulated by plasma concentrations of FVII or an equivalent concentration of FVIIa (10 nM). Neither thrombin nor other proteases involved in hemostasis were effective in stimulating IL-8 in these cells. Increased transcriptional activation of the IL-8 gene is responsible for increased expression of IL-8 in FVIIa-treated cells. PAR-2–specific antibodies fully attenuated TF-FVIIa–induced IL-8 expression. Additional in vitro experiments showed that TF-FVIIa promoted tumor cell migration and invasion, active site–inactivated FVIIa, and specific antibodies against TF, PAR-2, and IL-8 inhibited TF-FVIIa–induced cell migration. In summary, the studies described herein provide insight into how TF may contribute to tumor invasion. (Blood. 2004;103:3029-3037) |
| Databáze: | OpenAIRE |
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