Constitutive over-expression of transforming growth factor-alpha in rat liver epithelial cells leads to increased cell cycling without transformation
Autor: | J. Hanley-Hyde, Snorri S. Thorgeirsson, Thomas B. Tan, Lori L. Hampton, Pamela A. Marino, Raji Padmanabhan |
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Rok vydání: | 1994 |
Předmět: |
TGF alpha
Cellular differentiation Drug Resistance Gene Expression Mice Nude Biology Transfection Epithelium Cell Line Mice Epidermal growth factor Animals RNA Messenger Cell Cycle Neomycin Cell Biology General Medicine Cell cycle Transforming Growth Factor alpha Molecular biology Cell biology Rats ErbB Receptors Blotting Southern Cell Transformation Neoplastic Retroviridae Liver Cell culture Transforming growth factor beta 3 Stem cell Developmental Biology Transforming growth factor |
Zdroj: | In vitro cellulardevelopmental biology. Animal. (9) |
ISSN: | 1071-2690 |
Popis: | Over-expression of transforming growth factor-alpha (TGF-alpha) is consistently seen in spontaneous transformants of rat liver derived epithelial cells (RLE phi 13) and has been implicated in the transformation of other cultured cells. We have constitutively over-expressed TGF-alpha in RLE phi 13 cells, which are known to express epidermal growth factor receptors, to determine if TGF-alpha over-expression plays a role in transformation or differentiation, or both, of these cells. Early passage RLE phi 13 cells were infected with a replication-defective murine retrovirus that expresses both the full length coding sequence for human TGF-alpha and the neomycin-resistance gene. Integration of the transcriptionally active provirus and expression of TGF-alpha mRNA were confirmed. Neither morphologic transformation nor molecular evidence for differentiation was noted in TGF-alpha-producing clones. However, these clones did exhibit an accelerated growth rate, increased expression of several cell cycle related genes including mitotic cyclic B1, proliferating cell nuclear antigen, c-myc, and p53 as well as increased expression of the preneoplastic marker enzyme, glutathione-S-transferase. This suggests that over-expression of TGF-alpha results in increased cell cycling, and that subsequent events must be necessary for cellular transformation or differentiation or both. |
Databáze: | OpenAIRE |
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