Monitoring airway mucus flow and ciliary activity with optical coherence tomography
| Autor: | Raghav K. Chhetri, Amy L. Oldenburg, Brian Button, David B. Hill |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty 01 natural sciences Cystic fibrosis 010309 optics 03 medical and health sciences Optical coherence tomography 0103 physical sciences ocis:(170.4500) Optical coherence tomography Medicine 030304 developmental biology 0303 health sciences ocis:(110.4153) Motion estimation and optical flow medicine.diagnostic_test business.industry Cilium ocis:(170.2655) Functional monitoring and imaging respiratory system medicine.disease Mucus Atomic and Molecular Physics and Optics 3. Good health ocis:(110.6150) Speckle imaging Respiratory epithelium Optical Coherence Tomography ocis:(170.3880) Medical and biological imaging business Airway Preclinical imaging Ex vivo Biotechnology ocis:(110.0113) Imaging through turbid media |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Popis: | Muco-ciliary transport in the human airway is a crucial defense mechanism for removing inhaled pathogens. Optical coherence tomography (OCT) is well-suited to monitor functional dynamics of cilia and mucus on the airway epithelium. Here we demonstrate several OCT-based methods upon an actively transporting in vitro bronchial epithelial model and ex vivo mouse trachea. We show quantitative flow imaging of optically turbid mucus, semi-quantitative analysis of the ciliary beat frequency, and functional imaging of the periciliary layer. These may translate to clinical methods for endoscopic monitoring of muco-ciliary transport in diseases such as cystic fibrosis and chronic obstructive pulmonary disease (COPD). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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