Secondary dengue virus infections during the 2009 outbreak in Buenos Aires
| Autor: | E. Tittarelli, Laura E. Valinotto, P. R. Barrero, A. S. Mistchenko |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
viruses Secondary infection Suspected dengue Dengue virus medicine.disease_cause Serology Ciencias Biológicas 03 medical and health sciences Medicine DENGUE VIRUS SECONDARY INFECTIONS business.industry Public Health Environmental and Occupational Health Outbreak Serum samples Virology VIRAL LOAD 030104 developmental biology Infectious Diseases Clinical diagnosis Immunology Parasitology business Virología Viral load CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Tropical Medicine & International Health. 21:28-32 |
| ISSN: | 1360-2276 |
| DOI: | 10.1111/tmi.12619 |
| Popis: | Objectives: To evaluate the occurrence of secondary dengue virus (DENV) infections during the 2009 outbreak in a non-endemic area. Viral loads were evaluated in serum from acute-phase patients, comparing primary and secondary infection. Methods: Serum samples from patients with clinical diagnosis of suspected dengue were referred to the Virology Laboratory at 'Ricardo Gutiérrez' Children's Hospital. Dengue-positive samples were classified as primary or secondary DENV infections through serological methods (anti-DENV IgM and IgG). Viral loads were measured by quantitative real-time PCR (qRT-PCR) in samples obtained in the first 5 days of infection. Statistical analyses were performed to evaluate factors that might correlate with differences in the viral load of primary or secondary infection. Results: A total of 229 DENV cases were confirmed; among them, 22.7% were secondary infections. No significant differences were found between the viral load of primary and secondary infections. Conclusion: We detected a high percentage of secondary DENV infections in a non-endemic area; this finding might correspond to socio-demographic characteristics of the group under study or indicate a previous cryptic DENV circulation causing inapparent infections. Fil: Tittarelli, Estefanía. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Barrero, Paola Roxana. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mistchenko, Alicia Susana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Laboratorio de Virología; Argentina Fil: Valinotto, Laura Elena. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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