Aging Increases Susceptibility to High Fat Diet-Induced Metabolic Syndrome in C57BL/6 Mice: Improvement in Glycemic and Lipid Profile after Antioxidant Therapy

Autor: Luiza A. Rabelo, Lucas José Sá Da Fonseca, Salete Smaniotto, Valéria Nunes-Souza, Glaucevane Da Silva Guedes, Cheila Juliana César-Gomes
Rok vydání: 2016
Předmět:
Blood Glucose
0301 basic medicine
Aging
medicine.medical_specialty
Article Subject
medicine.medical_treatment
Diet
High-Fat
Biochemistry
Antioxidants
Cyclic N-Oxides
Mice
03 medical and health sciences
chemistry.chemical_compound
Insulin resistance
Non-alcoholic Fatty Liver Disease
Internal medicine
Nonalcoholic fatty liver disease
medicine
Animals
Insulin
lcsh:QH573-671
Triglycerides
Metabolic Syndrome
Glucose tolerance test
medicine.diagnostic_test
lcsh:Cytology
Superoxide Dismutase
Acetophenones
Cell Biology
General Medicine
Glucose Tolerance Test
Catalase
medicine.disease
Lipids
Mice
Inbred C57BL
Disease Models
Animal
030104 developmental biology
Endocrinology
Liver
chemistry
Apocynin
cardiovascular system
Spin Labels
Lipid Peroxidation
Metabolic syndrome
Steatosis
Lipid profile
Research Article
Zdroj: Oxidative Medicine and Cellular Longevity
Oxidative Medicine and Cellular Longevity, Vol 2016 (2016)
ISSN: 1942-0994
1942-0900
DOI: 10.1155/2016/1987960
Popis: Nonalcoholic fatty liver disease (NAFLD) has been considered a novel component of the metabolic syndrome (MetS), with the oxidative stress participating in its progression. This study aimed to evaluate the metabolic profile in young and old mice with MetS, and the effects of apocynin and tempol on glycemic and lipid parameters. Young and old C57BL/6 mice with high fat diet- (HFD-) induced MetS received apocynin and tempol 50 mg·kg−1/day in their drinking water for 10 weeks. After HFD, the young group showed elevated fasting glucose, worsened lipid profile in plasma, steatosis, and hepatic lipid peroxidation. Nevertheless, the old group presented significant increase in fasting insulin levels, insulin resistance, plasma and hepatic lipid peroxidation, and pronounced steatosis. The hepatic superoxide dismutase and catalase activity did not differ between the groups. Tempol and apocynin seemed to prevent hepatic lipid deposition in both groups. Furthermore, apocynin improved glucose tolerance and insulin sensitivity in old mice. In summary, old mice are more susceptible to HFD-induced metabolic changes than their young counterparts. Also, the antioxidant therapy improved insulin sensitivity and glucose tolerance, and in addition, apocynin seemed to prevent the HFD-induced hepatic fat deposition, suggesting an important role of oxidative stress in the induction of NAFLD.
Databáze: OpenAIRE
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