Application of Hydrogel in Reconstruction Surgery: Hydrogel/Fat Graft Complex Filler for Volume Reconstruction in Critical Sized Muscle Defects
| Autor: | Wing Yuk Ip, Y. F. Lui |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Filler (packaging) Materials science Article Subject lcsh:Medicine Adipose tissue Biocompatible Materials 030230 surgery Transplantation Autologous General Biochemistry Genetics and Molecular Biology Fat pad Reconstruction surgery Rats Sprague-Dawley Cicatrix Mice 03 medical and health sciences 0302 clinical medicine Tissue engineering 3T3-L1 Cells In Situ Nick-End Labeling medicine Animals Volume reconstruction Pentobarbital Tissue Engineering General Immunology and Microbiology Muscles lcsh:R Graft Survival Hydrogels General Medicine Plastic Surgery Procedures Rats Surgery Transplantation surgical procedures operative Adipose Tissue 030220 oncology & carcinogenesis Self-healing hydrogels Female Collagen Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2016 (2016) |
| Popis: | Autogenic fat graft usually suffers from degeneration and volume shrinkage in volume reconstruction applications. How to maintain graft viability and graft volume is an essential consideration in reconstruction therapies. In this presented investigation, a new fat graft transplantation method was developed aiming to improve long term graft viability and volume reconstruction effect by incorporation of hydrogel. The harvested fat graft is dissociated into small fragments and incorporated into a collagen based hydrogel to form a hydrogel/fat graft complex for volume reconstruction purpose. In vitro results indicate that the collagen based hydrogel can significantly improve the survivability of cells inside isolated graft. In a 6-month investigation on artificial created defect model, this hydrogel/fat graft complex filler has demonstrated the ability of promoting fat pad formation inside the targeted defect area. The newly generated fat pad can cover the whole defect and restore its original dimension in 6-month time point. Compared to simple fat transplantation, this hydrogel/fat graft complex system provides much improvement on long term volume restoration effect against degeneration and volume shrinkage. One notable effect is that there is continuous proliferation of adipose tissue throughout the 6-month period. In summary, the hydrogel/fat graft system presented in this investigation demonstrated a better and more significant effect on volume reconstruction in large sized volume defect than simple fat transplantation. |
| Databáze: | OpenAIRE |
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