STUDIES ON THE INCREASE OF VACCINE VIRUS IN CULTURED HUMAN CELLS BY MEANS OF THE FLUORESCENT ANTIBODY TECHNIQUE
| Autor: | Wilbur Fiske Noyes, Barbara K. Watson |
|---|---|
| Rok vydání: | 1955 |
| Předmět: |
Vaccines
biology medicine.diagnostic_test Vaccination Immunology Fluorescent Antibody Technique Immunofluorescence Molecular biology Antibodies Fluorescence Article Cell Line Staining medicine.anatomical_structure Antigen Cytoplasm medicine biology.protein Humans Immunology and Allergy Antigens Nuclear membrane Antibody Nucleus Pan-T antigens |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.102.3.237 |
| Popis: | The specific detection of the antigens of vaccine virus in cultured human cells by means of fluorescent antibody is reported. The antigenic material occurred in the cytoplasm in progressively increasing amounts and in the early stages was in close contact with the nuclear membrane. The antigens were present in a finely particulate form, either as foci or loosely spread out, up to late stages of infection, at which time the staining assumed a homogeneous form due either to close packing of the particles or to soluble antigen, or both. In late stages, homogeneous collections of antigen were observed in the nucleus. They may have resulted from diffusion of soluble antigen through the nuclear membrane. The antigens were observed in situations which suggest a new method of spread from one to another of these cells, namely by transmission through cytoplasmic connections. |
| Databáze: | OpenAIRE |
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