Distribution, Functional Expression, and Genetic Organization of Cif, a Phage-Encoded Type III-Secreted Effector from Enteropathogenic and EnterohemorrhagicEscherichia coli
| Autor: | Monique Kérourédan, Herbert Schmidt, Estelle Loukiadis, Tadasuke Ooka, Sylvia Herold, Yoshitoshi Ogura, Rika Nobe, Tetsuya Hayashi, Hubert Brugère, Stefano Morabito, Eric Oswald, C. Tramuta |
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| Přispěvatelé: | Inconnu, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées |
| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial [SDV]Life Sciences [q-bio] Prophages Molecular Sequence Data Population Virulence Genetics and Molecular Biology Biology medicine.disease_cause Microbiology Bacteriophage Enteropathogenic Escherichia coli 03 medical and health sciences medicine Bacteriophages education Molecular Biology Escherichia coli ComputingMilieux_MISCELLANEOUS Phylogeny Prophage 030304 developmental biology Genetics 0303 health sciences education.field_of_study 030306 microbiology Effector Escherichia coli Proteins biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Phenotype Genes Bacterial Enterohemorrhagic Escherichia coli Locus of enterocyte effacement |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 2008, 190 (1), pp.275-285. ⟨10.1128/JB.00844-07⟩ |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.00844-07 |
| Popis: | EnteropathogenicEscherichia coli(EPEC) and enterohemorrhagicE. coli(EHEC) inject effector proteins into host cells via a type III secretion system encoded by the locus of enterocyte effacement (LEE). One of these effectors is Cif, encoded outside the LEE by a lambdoid prophage. In this study, we demonstrated that the Cif-encoding prophage of EPEC strain E22 is inducible and produces infectious phage particles. We investigated the distribution and functional expression of Cif in 5,049E. colistrains of human, animal, and environmental origins. A total of 115E. coliisolates from diverse origins and geographic locations carriedcif. The presence ofcifwas tightly associated with the LEE, since all thecif-positive isolates were positive for the LEE. These results suggested that the Cif-encoding prophages have been widely disseminated within the natural population ofE. colibut positively selected within the population of LEE-positive strains. Nonetheless, 66% ofcif-positiveE. colistrains did not induce a typical Cif-related phenotype in eukaryotic cells due to frameshift mutations or insertion of an IS element in thecifgene. The passenger region of the prophages carryingcifwas highly variable and showed various combinations of IS elements and genes coding for other effectors such asnleB,nleC,nleH,nleG,espJ, andnleA/espI(some of which were also truncated). This diversity and the presence of nonfunctional effectors should be taken into account to assess EPEC and EHEC pathogenicity and tropism. |
| Databáze: | OpenAIRE |
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