Molecular Evidence of Organic Ion Transporters in the Rat Adrenal Cortex with Adrenocorticotropin-Regulated Zonal Expression
| Autor: | E. Beéry, Stefan R. Bornstein, Gerhard Burckhardt, Andrew Bahn, Yohannes Hagos, P. Middel, Hermann Koepsell, H. S. Willenberg, Jürgen Steffgen, Gerhard A. Müller |
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| Rok vydání: | 2003 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.medical_treatment Organic Anion Transporters Sodium-Independent Biology Polymerase Chain Reaction Xenopus laevis 03 medical and health sciences Organic Anion Transport Protein 1 0302 clinical medicine Endocrinology Adrenocorticotropic Hormone Zona fasciculata Internal medicine medicine Animals Tissue Distribution RNA Messenger Rats Wistar Steroid 11-beta-hydroxylase In Situ Hybridization Hypophysectomy 030304 developmental biology Hydrocortisone 0303 health sciences Organic cation transport proteins Adrenal cortex Rats Steroid hormone medicine.anatomical_structure Zona glomerulosa 030220 oncology & carcinogenesis Adrenal Cortex Oocytes biology.protein Zona reticularis medicine.drug |
| Zdroj: | Endocrinology. 144:4519-4526 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/en.2002-221001 |
| Popis: | Experimental evidence suggested that secretion of steroid hormones from adrenocortical cells involves carrier-mediated transport: Cortisol release from, and uptake of p-[3H]aminohippurate into, bovine adrenocortical cells showed properties of the renal p-[3H]aminohippurate/anion exchanger OAT1. Other poly-specific transporters such as organic anion-transporting polypeptides (oatps) and organic cation transporters (OCTs) could also be involved in steroid hormone release. A homology-cloning procedure was established to detect these transporters in rat adrenal gland cDNA. PCR revealed the presence of OAT1, oatp1, oatp2, and oatp3. In situ hybridization localized OAT1 in the outer zona fasciculata, oatp3 in the zona glomerulosa, and oatp1 and oatp2 in the inner zona fasciculata and outer zona reticularis. An OCT2-specific probe produced signals in the zona glomerulosa and outer zona fasciculata. Pretreatment of rats with ACTH increased the expression of OAT1 mRNA that spread to all zones, and hypophysectomy strongly decreased it. A less pronounced regulation was detected for OCT2 and oatp3. Specific antibodies confirmed the localization of OAT1 in the outer zona fasciculata, supporting a possible role of OAT1 in cortisol release. The zonated distribution of transporters furthermore suggest that oatp1-3 and OCT2 may be important for the endocrine function of rat adrenocortical cells. |
| Databáze: | OpenAIRE |
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