Dexamethasone protects the glycocalyx on the kidney microvascular endothelium during severe acute pancreatitis
| Autor: | Weina Lu, Zhong-Yan Liang, Qinghui Fu, Tingbo Liang, Jian Zhang, Yun Zhang, Shao-yang Zhang, Wen-Qiao Yu, Shuiqiao Fu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty medicine.medical_treatment Intraperitoneal injection H&E stain Enzyme-Linked Immunosorbent Assay Glycocalyx Kidney Protective Agents General Biochemistry Genetics and Molecular Biology Dexamethasone Article Microcirculation 03 medical and health sciences Mice 0302 clinical medicine medicine Animals Edema General Pharmacology Toxicology and Pharmaceutics General Veterinary business.industry Tumor Necrosis Factor-alpha General Medicine medicine.disease Mice Inbred C57BL Perfusion Disease Models Animal 030104 developmental biology medicine.anatomical_structure Pancreatitis 030220 oncology & carcinogenesis Acute Disease Acute pancreatitis Endothelium Vascular business medicine.drug |
| Zdroj: | Journal of Zhejiang University. Science. B. 20(4) |
| ISSN: | 1862-1783 |
| Popis: | Objective: This study demonstrated that dexamethasone (DEX) protects the endothelial glycocalyx from damage induced by the inflammatory stimulus tumor necrosis factor-α (TNF-α) during severe acute pancreatitis (SAP), and improves the renal microcirculation. Methods: Ninety mice were evenly divided into 3 groups (Sham, SAP, and SAP+DEX). The SAP mice model was established by ligature of pancreatic duct and intraperitoneal injection of cerulein. Renal perfusion and function, and morphological changes of the glycocalyx were evaluated by laser Doppler velocimetry, electron microscopy, and histopathology (hematoxylin and eosin (H&E) staining), respectively. Serum levels of syndecan-1 and TNF-α were assessed by enzyme-linked immunosorbent assay (ELISA). The protective effects of dexamethasone on the glycocalyx and renal microcirculation were evaluated. Results: Significantly high levels of serum TNF-α were detected 3 h after the onset of SAP. These levels might induce degradation of the glycocalyx and kidney hypoperfusion, resulting in kidney microcirculation dysfunction. The application of dexamethasone reduced the degradation of the glycocalyx and improved perfusion of kidney. Conclusions: Dexamethasone protects the endothelial glycocalyx from inflammatory degradation possibly initiated by TNF-α during SAP. This is might be a significant discovery that helps to prevent tissue edema and hypoperfusion in the future. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink