High-resolution particle size analysis in biotechnology process control

Autor:	John C. Thomas, Mark A. Snoswell, Jean Francois Hamel, Anton P. J. Middelberg
Rok vydání:	1991
Předmět:	Inclusion Bodies Centrifuge Chromatography Chemistry High resolution Proteins Centrifugation Inclusion bodies Particle-size distribution Separation method Process control Insoluble protein Particle Size Biotechnology
Zdroj:	Biotechnology progress. 7(4)
ISSN:	8756-7938
Popis:	Many industrially important proteins can now be expressed intracellularly as insoluble protein inclusion bodies. In production, large-scale centrifugation is commonly used to separate and recover the inclusion bodies. Recovery efficiency depends critically on the centrifuge feed rate, which must be optimized to minimize production costs. We have used a disc centrifuge photosedimentometer to make high-resolution measurements of the particle size distribution (PSD) of the supernatant during the production of porcine somatotropin (pST) inclusion bodies. These measurements readily monitor the breakthrough of inclusion bodies into the supernatant and allow the centrifugation operation to be optimized.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a549c7f8c48ee739a9d2b7fd9e85f283 https://pubmed.ncbi.nlm.nih.gov/1369330 Zobrazit plný text záznamu Plný text