Cyclosporin A Affects Signaling Events Differentially in Human Gingival Fibroblasts
| Autor: | H. Bharath, A. Bostrom, A. S. Narayanan, A. Saulewicz |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
0301 basic medicine
Cell signaling MAP Kinase Kinase 4 T-Lymphocytes p38 mitogen-activated protein kinases Gingiva Lymphocyte Activation p38 Mitogen-Activated Protein Kinases 03 medical and health sciences 0302 clinical medicine Western blot Cyclosporin a medicine Humans Extracellular Signal-Regulated MAP Kinases General Dentistry Transcription factor Cells Cultured Mitogen-Activated Protein Kinase Kinases Platelet-Derived Growth Factor Mitogen-Activated Protein Kinase 3 NFATC Transcription Factors medicine.diagnostic_test biology Tumor Necrosis Factor-alpha Kinase JNK Mitogen-Activated Protein Kinases NF-kappa B Nuclear Proteins NFAT 030206 dentistry Fibroblasts Molecular biology DNA-Binding Proteins Transcription Factor AP-1 030104 developmental biology Cyclosporine Cancer research biology.protein Immunosuppressive Agents Platelet-derived growth factor receptor Signal Transduction Transcription Factors |
| Zdroj: | Journal of Dental Research. 84:532-536 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/154405910508400609 |
| Popis: | Gingival overgrowth is a common side-effect of the administration of cyclosporin A (CSA), phenytoin, and calcium blockers. To identify the signaling mechanisms possibly involved in the overgrowth, we examined how CSA affects the activities of MAP kinases and transcription factors in human gingival fibroblasts (HGF). The HGF were treated with CSA and TNF-alpha or PDGF. DNA-binding activity of NFAT, NFkappaB, and AP-1 transcription factors was determined by gel shift assay, and JNK, p38, and ERK1 and ERK2 activation was assessed by Western blot analysis of immunoprecipitates. The CSA inhibited NFAT, NFkappaB, and p38 and JNK activities; however, ERK1 and ERK2 were not affected significantly. AP-1 activity increased approximately 4.5-fold. Our results indicate that CSA affects signaling molecules in HGF differently from other cell types, and that a CSA-induced increase in AP-1 activity may affect the expression of fibrogenic molecules in gingiva and promote gingival overgrowth. |
| Databáze: | OpenAIRE |
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