Surface patterns of insulin fibrils revealed by time-resolved spectroscopy measurements of fluorescent probes
| Autor: | A. Justyniarski, Alexander Mikhailovsky, Piotr Hanczyc, J. Kim, Magdalena I. Ivanova |
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| Rok vydání: | 2018 |
| Předmět: |
Photoluminescence
Amyloid Biophysics 02 engineering and technology General Chemistry macromolecular substances 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Fibril 01 natural sciences Biochemistry Fluorescence Atomic and Molecular Physics and Optics Fluorescence spectroscopy 0104 chemical sciences chemistry.chemical_compound chemistry Thioflavin Time-resolved spectroscopy 0210 nano-technology Spectroscopy |
| Zdroj: | Journal of Luminescence |
| DOI: | 10.5281/zenodo.3834315 |
| Popis: | Amyloid fibrils are a hallmark of neurodegeneration. The structural diversity of amyloids necessitates sensitive methods and probes that can be reliably used to characterize them. Here, we study insulin fibrils and its polymorphs seeded with LVEALYL peptide in context of probing the surface patterns using Thioflavin T (ThT) and polythiophene derivative – Poly[2-(3-thienyl)ethoxy-4-butylsulfonate] (PTEBS) polymer. We investigated the dynamics and lifetimes of these two probes using time-resolved absorption and fluorescence spectroscopy. The photoluminescence emission lifetimes of the probes showed different relaxation times in the presence of structurally different amyloid fibrils. However, only PTEBS revealed sensitivity to the surface patterns of the fibrils that was explained by uneven charge distribution and exposure of different amino acids to the fibril surface where probe is interacting with the fibril. We find that PTEBS binding to distinct amyloid surfaces causes perturbation of the main chain of the polymer creating new conditions for energy distribution in the excited states that favors formation of various emitting species. The results indicate that PTEBS and ThT are comparable in terms of recognition of fibril polymorphs whereas polymer probe provides additional dimension to study fibrils surface patterns that can be revealed using time resolved spectroscopy. |
| Databáze: | OpenAIRE |
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