Evaluation of anti-inflammatory, analgesic and TNF-α inhibition (upon RAW 264.7 cell line) followed by the selection of extract (leaf and stem) with respect to potency to introduce anti-oral-ulcer model obtained from Olax psittacorum (Lam.) Vahl in addition to GC-MS illustration
| Autor: | Raja Majumder, Lopamudra Adhikari, Jinamitra Sahu, Moonmun Dhara |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.drug_class Analgesic Anti-Inflammatory Agents Drug Evaluation Preclinical Gas Chromatography-Mass Spectrometry Anti-inflammatory Mice 03 medical and health sciences 0302 clinical medicine Diclofenac Pharmacokinetics Drug Discovery medicine Animals Potency Antipyretic Rats Wistar Oral Ulcer Pain Measurement 030304 developmental biology Pharmacology Analgesics 0303 health sciences Dose-Response Relationship Drug Plant Stems Olax psittacorum biology Traditional medicine Plant Extracts Tumor Necrosis Factor-alpha Chemistry Anti-Ulcer Agents biology.organism_classification Rats Plant Leaves RAW 264.7 Cells 030220 oncology & carcinogenesis Toxicity Female Olacaceae medicine.drug |
| Zdroj: | Journal of Ethnopharmacology. 263:113146 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2020.113146 |
| Popis: | Olax psittacorum (Lam.) Vahl., belongs to family olacaceae claimed as an "Issan folk medicine" portray the ethnomedicinal value like curative property of infection in the urinary tract, analgesic, antipyretic, skin-ulcer, antianemic (bark) as well as food additives (leaves). Research articles have proven the presence of anti-swelling property, laxative action, and antiviral activity against poliovirus moreover, the antioxidant property too.Evaluation of antiulcer property (induced within the oral mucosa) of the extract selected amongst two extracts based upon better property towards the ability of anti-inflammatory and analgesia through the in-vivo model as well as the inhibitory property of TNF-α (cell line RAW264.7). To justify the presence of activity extracts were introduced for GC-MS investigation.Methanolic extracts (leaf; LME and stem; SME) were collected through maceration and introduced to carrageenan-induced paw edema to evaluate the anti-inflammatory activity and formalin-induced as well as tail-flick in-vivo models to evaluate the analgesic property. Anti-oral ulcer property was analyzed through the acetic-acid induced in-vivo model. The cytotoxicity was performed on mouse macrophages and fibroblast cells to find a toxic concentration of test substances and to evaluate their modulatory effect of TNF-α inhibition property against LPS induced toxicity.As compared to diclofenac (100 mg/kg) only LME and SME 200 mg/kg dose group have insignificant (P 0.05) difference and P-values are 0.99 and 0.88 respectively. From the overall outcome, it can be concluded that compared to the diclofenac (100 mg/kg) group from 4th hours onwards LME (200 mg/kg) group was able to sustain the inflammation so similar. According to statistical consideration, LME (200 mg/kg) dose has also shown better results in formalin-induced analgesia as well as tail-flick. Cytotoxicity (CTCThe overall outcomes of the study encapsulate that LME extract with a dose of 200 mg/kg/bw will be a good choice to overcome the above-cited ailments. Further studies upon this plant are needed to establish its importance in the human society through quantitative isolation of the metabolites and their pharmacokinetic as well as pharmacodynamic evaluation to establish the proper pathway of action. |
| Databáze: | OpenAIRE |
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