Defective Chemokine Production in T-Leukemia Cell Lines and its Possible Functional Role
| Autor: | Anna Ivanoff, Karl-Gösta Sundqvist, Jyrki Ivanoff |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
Chemokine Leukemia T-Cell extracellular matrix medicine.medical_treatment Immunology chemokines cell motility CCL7 Extracellular matrix Cell Movement Tumor Cells Cultured medicine Humans CX3CL1 T-lymphocytes biology cell adhesion Chemotaxis Cell biology Fibronectin Cytokine biology.protein Pseudopodia lcsh:RC581-607 Research Article Developmental Biology |
| Zdroj: | Clinical and Developmental Immunology, Vol 7, Iss 2-4, Pp 67-75 (2000) Developmental Immunology |
| ISSN: | 1044-6672 |
| DOI: | 10.1155/2000/28085 |
| Popis: | Peripheral blood lymphocytes and T-cell clones produced nanogram quantities of the chemokines RANTES, MIP-1alpha, MIP-1beta, MCP-1, IL-8 and GRO-alpha as well as the motogenic cytokine HGF. In contrast, various T-leukemia cell lines at different stages of differentiation did not produce the same chemokines/cytokines. In order to study the possible functional importance of the poor chemokine production different T-cell lines were compared with respect to development of motile forms and migration on extracellular matrix components in the absence and presence of various chemokines. RANTES, MIP-1alpha, MIP-1beta, IL-8, GRO-alpha and lymphotactin did not augment the development of motile forms including the size and appearance of the pseudopodia activity of the T-leukemia cell lines. The T-cell lines migrated spontaneously on/to fibronectin in a Boyden chamber assay system. Chemokines augmented the migration of the T-leukemia cell lines on fibronectin in the Boyden system in a chemotactic fashion with peak responses at 10 to 50 ng/ml. Thus, the production of chemokines is defective in neoplastic T-lymphocytes. The defective chemokine production does not seem to play any major role for the basic locomotor capacity of the cells but may modulate the responsiveness to exogenous chemokines. |
| Databáze: | OpenAIRE |
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