Response properties of whisker-associated primary afferent neurons following infraorbital nerve transection with microsurgical repair in adult rats
| Autor: | Kia M. Washington, Daniel J. Simons, Rami R. Zanoun, George E. Carvell, Bo Xiao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Refractory Period Electrophysiological Physiology Stimulation Rats Sprague-Dawley 03 medical and health sciences Trigeminal ganglion Infraorbital nerve 0302 clinical medicine Peripheral Nerve Injuries Evoked Potentials Somatosensory Nervous System Pathophysiology Reaction Time Animals Medicine Neurons Afferent Trigeminal nerve business.industry General Neuroscience Nerve injury Adaptation Physiological Rats Peripheral Ganglion 030104 developmental biology medicine.anatomical_structure Trigeminal Ganglion Vibrissae Peripheral nerve injury medicine.symptom business Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neurophysiology. 115:1458-1467 |
| ISSN: | 1522-1598 0022-3077 |
| DOI: | 10.1152/jn.00970.2015 |
| Popis: | The rodent whisker/trigeminal system, characterized by high spatial and temporal resolution, provides an experimental model for developing new therapies for improving sensory functions of damaged peripheral nerves. Here, we use controlled whisker stimulation and single-unit recordings of trigeminal ganglion cells to examine in detail the nature and time course of functional recovery of mechanoreceptive afferents following nerve transection with microsurgical repair of the infraorbital nerve (ION) branch of the trigeminal nerve in adult rats. Response measures include rapid vs. slow adaptation, firing rate, interspike intervals, latency, and angular (directional) tuning. Whisker-evoked responses, readily observable by 3 wk post-transection, recover progressively for at least the next 5 wk. All cells in transected animals, as in control cases, responded to deflections of single whiskers only, but topography within the ganglion was clearly disrupted. The time course and extent of recovery of quantitative response measures were receptor dependent. Cells displaying slowly adapting (SA) properties recovered more quickly than rapidly adapting (RA) populations, and for some response measures—notably evoked firing rates—closely approached or attained control levels by 8 wk post-transection. Angular tuning of RA cells was slightly better than control units, whereas SA tuning did not differ from control values. Nerve conduction times and refractory periods, examined separately using electrical stimulation of the ION, were slower than normal in all transected animals and poorly reflected recovery of whisker-evoked response latencies and interspike intervals. Results underscore the need for multiple therapeutic strategies that target different aspects of functional restitution following peripheral nerve injury. |
| Databáze: | OpenAIRE |
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