The Composition of Metals Bound to Class III Metallothionein (Phytochelatin and Its Desglycyl Peptide) Induced by Various Metals in Root Cultures of Rubia tinctorum
Autor: | H. Kubota, T. Maitani, T. Yamada, K. Sato |
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Rok vydání: | 1996 |
Předmět: |
chemistry.chemical_classification
Rubia tinctorum Liaison biology Physiology Stereochemistry Peptide Plant Science biology.organism_classification Metal chemistry.chemical_compound Biosynthesis chemistry visual_art Genetics visual_art.visual_art_medium Metallothionein Composition (visual arts) Phytochelatin Nuclear chemistry Research Article |
Zdroj: | Plant physiology. 110(4) |
ISSN: | 1532-2548 |
Popis: | The induction of phytochelatins (PCs) and their desglycyl peptides (both are referred to as class III metallothionein [CIIIMT]) by exposure to various metals (Ag+, As3+, As5+, Cd2+, Cu2+, Ga3+, Hg2+, In3+, Ni2+, Pb2+, Pd2+, Se4+, and Zn2+) and the metal composition in the CIIIMTs were investigated in root cultures of Rubia tinctorum L. All of these metal species induced PCs to various degrees when analyzed by the postcolumn derivatization high-performance liquid chromatography method. The desglycyl peptides of PCs often were also present. However, only Ag, Cd, and Cu were bound to the CIIIMTs that they induced when analyzed by the high-performance liquid chromatography-inductively coupled plasma-atomic emission spectrometry method. Cu was also bound to the CIIIMTs induced by Ag+, As3+, and Cd2+. After Ag+ exposure, an Fe peak that may be of Fe-CIIIMT was also observed. However, most of the metal species studied were not bound to the CIIIMTs that they induced. |
Databáze: | OpenAIRE |
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