A ratiometric ESIPT probe based on 2-aza-Cope rearrangement for rapid and selective detection of formaldehyde in living cells
| Autor: | Guanling Zeng, Tingting Quan, Tongsheng Chen, Huaiting Pang, Zhenhao Liang |
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| Rok vydání: | 2022 |
| Předmět: |
Detection limit
Photons Stimulation Endogeny medicine.disease_cause Biochemistry Fluorescence Analytical Chemistry Chemical kinetics Metabolic pathway Formaldehyde MCF-7 Cells Electrochemistry Aza-Cope rearrangement medicine Biophysics Humans Environmental Chemistry Spectroscopy Oxidative stress Fluorescent Dyes HeLa Cells |
| Zdroj: | The Analyst. 147:252-261 |
| ISSN: | 1364-5528 0003-2654 |
| Popis: | Formaldehyde (FA) is a crucial reactive signaling molecule participating in epigenetic and metabolic pathway. Whereas abnormally elevated levels of FA are implicated in various diseases spanning from tumors to neurodegenerative disorders. Although highly selective for FA, current 2-aza-Cope-based fluorescent probes leave room for improvement because their relatively slow reaction kinetics (1-9 hours for responsive time) hinder the capability to track biological transient FA. Herein, we presented a ratiometric fluorescent probe, FormAFP, based on excited state intramolecular photon transfer (ESIPT) for rapid (within 10 min), selective (above 70-fold over other RCS) and sensitive (240-times fluorescence enhancement with 66 nM of detection limit) detection of FA via 2-aza-cope rearrangement. The probe also displayed fast response ( |
| Databáze: | OpenAIRE |
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