Cell Adhesion on Silicon Nanowires
| Autor: | Damiano Cassese, Laura Andolfi, Anna Murello, Marco Lazzarino |
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| Rok vydání: | 2014 |
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| Zdroj: | Biophysical journal info:cnr-pdr/source/autori:Murello, Anna; Andolfi, Laura; Cassese, Damiano; Lazzarino, Marco/titolo:Cell Adhesion on Silicon Nanowires/doi:/rivista:Biophysical journal (Print)/anno:2014/pagina_da:389A/pagina_a:389A/intervallo_pagine:389A–389A/volume:106 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/j.bpj.2013.11.2201 |
| Popis: | Understanding nanostructured materials - cell interactions is essential for the development of innovative substrates for cell culture, differentiation and precise electrical stimulation. In this framework, silicon nanowires (SiNWs), characterized by high aspect ratio and a broad range of mechanical, optical and electrical properties, are interesting nanomaterials for innovative cellular engineering. We investigated and quantified, using single cell force spectroscopy (SCFS), the interaction of murine embryonic fibroblast with two mechanically different SiNW substrates, and two reference substrates, collagen gel which is generally used for cell culture, and flat glass which has the same chemical reactivity of SiNWs. We observed comparable adhesion values on SiNWs substrates and on gel substrate, while flat glass generate a 10-times weaker interaction. A closer analysis of the adhesion curves revealed significant differences between soft and hard SiNW, suggesting that a different cell membrane organization is developed depending on the mechanical and geometrical properties of the substrate. In conclusion SiNW substrates are compatible with cell engineering and thanks to their high range of mechanical and geometric properties, a fine tuning of cellular response can be achieved.Figure 1: representative SCFS curves for rigid NWs, flexible NWs and collagen-coated coverslips.View Large Image | View Hi-Res Image | Download PowerPoint Slide |
| Databáze: | OpenAIRE |
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