Alloxan action on glucose metabolism in cultured fibroblasts. I. Stimulation and inhibition of glucose utilization
| Autor: | R. M. Fields, Fukashi Ishibashi, Hideki Hidaka, P. H. Bennett, B. V. Howard |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Male
endocrine system medicine.medical_specialty endocrine system diseases Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Cell Biological Transport Active Stimulation Biology Carbohydrate metabolism chemistry.chemical_compound Physiology (medical) Internal medicine Alloxan medicine Humans Viability assay Cells Cultured Hexose transport Skin Pancreatic islets Insulin Methylglucosides nutritional and metabolic diseases Fibroblasts Kinetics Glucose medicine.anatomical_structure Endocrinology chemistry hormones hormone substitutes and hormone antagonists |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 240:E640-E644 |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.1981.240.6.e640 |
| Popis: | The influence of alloxan on mammalian cell glucose metabolism has been investigated using human diploid fibroblastic cells in culture. When cell monolayers were exposed to D-[14C]glucose, the presence of alloxan (0.31–1.87 mM) resulted initially in a dose-dependent enhancement of total cell glucose incorporation. This was observed within 2 min and declined by 6 min. After that time, alloxan inhibited glucose incorporation. When hexose transport was examined directly using glucose analogues, alloxan neither enhanced nor inhibited the uptake of 3-O-methylglucose or 2-deoxyglucose. Alloxan exerted no effect on cell permeability or cell viability. These results suggest that alloxan may directly influence cell glucose metabolism beyond the level of phosphorylation. The dual effect of alloxan on glucose incorporation may be related to the alloxan stimulation and subsequent inhibition of glucose-induced insulin release in pancreatic islets. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|