Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT
| Autor: | Maksim A Yakovlev, Dilworth Y. Parkinson, Keith C. Cheng, Yifu Ding, Spencer R Katz, Daniel J. Vanselow, Khai C. Ang, Victor A. Canfield, Yuxin Wang, Alex Y. Lin |
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| Rok vydání: | 2021 |
| Předmět: |
Silver Staining
QH301-705.5 Science ved/biology.organism_classification_rank.species Mutant Human skin Context (language use) micro-CT General Biochemistry Genetics and Molecular Biology Imaging Silver stain Melanin developmental biology Imaging Three-Dimensional 3D imaging cell biology Animals pigmentation Biology (General) Model organism whole-organism phenomics Zebrafish Melanins General Immunology and Microbiology biology ved/biology Chemistry General Neuroscience General Medicine X-Ray Microtomography Cell Biology Zebrafish Proteins biology.organism_classification zebrafish Chromatophore Phenotype Cell biology melanin Biochemistry Three-Dimensional Medicine Biomedical Imaging Biochemistry and Cell Biology Research Advance Whole Organism Developmental Biology |
| Zdroj: | eLife eLife, Vol 10 (2021) |
| ISSN: | 2050-084X |
| Popis: | We previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. The resulting images enabled whole-body, computational analyses of regional melanin content and morphology. Normalized micro-CT reconstructions of silver-stained fish consistently reproduced pigment patterns seen by light microscopy, and further allowed direct quantitative comparisons of melanin content across wild-type and mutant samples, including subtle phenotypes not previously noticed. Silver staining of melanin for micro-CT provides proof-of-principle for whole-body, 3D computational phenomic analysis of a specific cell type at cellular resolution, with potential applications in other model organisms and melanocytic neoplasms. Advances such as this in whole-organism, high-resolution phenotyping provide superior context for studying the phenotypic effects of genetic, disease, and environmental variables. |
| Databáze: | OpenAIRE |
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