Point-of-care detection of extracellular vesicles: Sensitivity optimization and multiple-target detection
| Autor: | María Yáñez-Mó, Myriam Oliveira-Rodríguez, María Carmen Blanco-López, Eva Cernuda-Morollón, Soraya López-Martín, Esther Serrano-Pertierra, Agustín Costa García |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Immunoconjugates Point-of-Care Systems Biomedical Engineering Biophysics Metal Nanoparticles Nanotechnology Biosensing Techniques Gold Colloid 02 engineering and technology Extracellular vesicles Tetraspanin 29 Tetraspanin 28 Extracellular Vesicles 03 medical and health sciences Limit of Detection Electrochemistry medicine Humans Magnetite Nanoparticles Point of care Immunoassay Detection limit Chromatography medicine.diagnostic_test Chemistry Vesicle Antibodies Monoclonal Equipment Design General Medicine 021001 nanoscience & nanotechnology 030104 developmental biology Colloidal gold Gold 0210 nano-technology Antibodies Immobilized Sensitivity (electronics) Biotechnology Lateral flow immunoassay |
| Zdroj: | Biosensors and Bioelectronics. 87:38-45 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2016.08.001 |
| Popis: | Extracellular vesicles (EVs) are membrane-bound nanovesicles delivered by different cellular lineages under physiological and pathological conditions. Although these vesicles have shown relevance as biomarkers for a number of diseases, their isolation and detection still has several technical drawbacks, mainly related with problems of sensitivity and time-consumed. Here, we reported a rapid and multiple-targeted lateral flow immunoassay (LFIA) system for the detection of EVs isolated from human plasma. A range of different labels (colloidal gold, carbon black and magnetic nanoparticles) was compared as detection probe in LFIA, being gold nanoparticles that showed better results. Using this platform, we demonstrated that improvements may be carried out by incorporating additional capture lines with different antibodies. The device exhibited a limit of detection (LOD) of 3.4×106EVs/µL when anti-CD81 and anti-CD9 were selected as capture antibodies in a multiple-targeted format, and anti-CD63 labeled with gold nanoparticles was used as detection probe. This LFIA, coupled to EVs isolation kits, could become a rapid and useful tool for the point-of-care detection of EVs, with a total analysis time of two hours. |
| Databáze: | OpenAIRE |
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