Three-Layered PCL Grafts Promoted Vascular Regeneration in a Rabbit Carotid Artery Model
Autor: | Lianyong Wang, Yong Guan, Yiwa Pan, Wenting Zheng, Yan Zhu, Jun Zhang, Kai Wang, Shaoyang Ma, Xin Zhou, Deling Kong, Ruming Liu, Qiang Zhao, Meifeng Zhu |
---|---|
Rok vydání: | 2016 |
Předmět: |
Vascular Endothelial Growth Factor A
0301 basic medicine Polymers and Plastics Endothelium Carotid Artery Common Polyesters Gene Expression Neovascularization Physiologic Bioengineering Nanotechnology macromolecular substances 02 engineering and technology Polyethylene Glycols Biomaterials 03 medical and health sciences chemistry.chemical_compound Tissue engineering Cell Movement In vivo Materials Chemistry medicine Animals Regeneration Chemokine CCL2 Tissue Engineering Tissue Scaffolds biology Chemistry Macrophages Regeneration (biology) technology industry and agriculture Electrochemical Techniques 021001 nanoscience & nanotechnology medicine.disease Electrospinning Blood Vessel Prosthesis Vascular endothelial growth factor Cellular infiltration 030104 developmental biology medicine.anatomical_structure biology.protein Female Rabbits Carotid Artery Injuries 0210 nano-technology Elastin Biotechnology Biomedical engineering |
Zdroj: | Macromolecular Bioscience. 16:608-618 |
ISSN: | 1616-5187 |
Popis: | In this study, a three layered poly (ε-caprolactone) (PCL) graft (tPCL) was fabricated by electrospinning PCL and electrospraying poly (ethylene oxide) (PEO), which has a thin dense inner layer, a loose middle layer, and a dense outer layer. Regular PCL grafts (rPCL) with only a dense layer were used as control. In vivo evaluation was performed in rabbit carotid artery. Enhanced cell infiltration, rapid regeneration of endothelium and smooth muscle layers, and increased elastin deposition were observed within the tPCL graft wall. After 3 months, tPCL grafts showed faster PCL degradation than the rPCL grafts. Infiltrated macrophages in the tPCL grafts secreted higher level of monocyte chemoattractant protein-1 (MCP-1) and vascular endothelial growth factor (VEGF) which enhanced vascular regeneration. In conclusion, the tPCL graft may be a useful vascular prosthesis and worth for further investigation. |
Databáze: | OpenAIRE |
Externí odkaz: |