Three-Layered PCL Grafts Promoted Vascular Regeneration in a Rabbit Carotid Artery Model

Autor: Lianyong Wang, Yong Guan, Yiwa Pan, Wenting Zheng, Yan Zhu, Jun Zhang, Kai Wang, Shaoyang Ma, Xin Zhou, Deling Kong, Ruming Liu, Qiang Zhao, Meifeng Zhu
Rok vydání: 2016
Předmět:
Vascular Endothelial Growth Factor A
0301 basic medicine
Polymers and Plastics
Endothelium
Carotid Artery
Common
Polyesters
Gene Expression
Neovascularization
Physiologic
Bioengineering
Nanotechnology
macromolecular substances
02 engineering and technology
Polyethylene Glycols
Biomaterials
03 medical and health sciences
chemistry.chemical_compound
Tissue engineering
Cell Movement
In vivo
Materials Chemistry
medicine
Animals
Regeneration
Chemokine CCL2
Tissue Engineering
Tissue Scaffolds
biology
Chemistry
Macrophages
Regeneration (biology)
technology
industry
and agriculture
Electrochemical Techniques
021001 nanoscience & nanotechnology
medicine.disease
Electrospinning
Blood Vessel Prosthesis
Vascular endothelial growth factor
Cellular infiltration
030104 developmental biology
medicine.anatomical_structure
biology.protein
Female
Rabbits
Carotid Artery Injuries
0210 nano-technology
Elastin
Biotechnology
Biomedical engineering
Zdroj: Macromolecular Bioscience. 16:608-618
ISSN: 1616-5187
Popis: In this study, a three layered poly (ε-caprolactone) (PCL) graft (tPCL) was fabricated by electrospinning PCL and electrospraying poly (ethylene oxide) (PEO), which has a thin dense inner layer, a loose middle layer, and a dense outer layer. Regular PCL grafts (rPCL) with only a dense layer were used as control. In vivo evaluation was performed in rabbit carotid artery. Enhanced cell infiltration, rapid regeneration of endothelium and smooth muscle layers, and increased elastin deposition were observed within the tPCL graft wall. After 3 months, tPCL grafts showed faster PCL degradation than the rPCL grafts. Infiltrated macrophages in the tPCL grafts secreted higher level of monocyte chemoattractant protein-1 (MCP-1) and vascular endothelial growth factor (VEGF) which enhanced vascular regeneration. In conclusion, the tPCL graft may be a useful vascular prosthesis and worth for further investigation.
Databáze: OpenAIRE
Externí odkaz: