Encapsulated Magnetic Nanoparticles as Supports for Proteins and Recyclable Biocatalysts
| Autor: | Byeong Su Kim, Aimee R. Herdt, T. Andrew Taton |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Conservation of Natural Resources
DNA Ligases Carboxylic acid Biomedical Engineering Pharmaceutical Science Nanoparticle Bioengineering Micelle Catalysis Magnetics Microscopy Electron Transmission medicine Organic chemistry T7 RNA polymerase Micelles Pharmacology chemistry.chemical_classification DNA ligase Bioconjugation Molecular Structure Organic Chemistry Proteins Combinatorial chemistry Spectrometry Fluorescence Enzyme chemistry Nanoparticles Magnetic nanoparticles Biotechnology medicine.drug |
| Zdroj: | Bioconjugate Chemistry. 18:183-189 |
| ISSN: | 1520-4812 1043-1802 |
| Popis: | This paper describes the bioconjugation of histidine-tagged enzymes and other proteins to the surface of composite "magnetomicelles" consisting of magnetic gamma-Fe2O3 nanoparticles encapsulated within cross-linked polystyrene-block-polyacrylate copolymer micelle shells. Free carboxylic acid groups on the magnetomicelle surface were converted to Cu2+-iminodiacetic acid (IDA) for protein capture. The conjugation of T4 DNA ligase and enhanced green fluorescent protein to magnetomicelles revealed that proteins were captured with a high surface density and could be magnetically separated from reaction mixtures and subsequently released from the nanoparticle surface. Additionally, bioconjugation of T7 RNA polymerase yielded a functional enzyme that maintained its biological activity and could be recycled for up to three subsequent transcription reactions. We propose that protein-magnetomicelle bioconjugates are effective for protein bioseparation and enzymatic recycling and further strengthen the idea that nanoparticle surfaces have utility in protein immobilization. |
| Databáze: | OpenAIRE |
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