Augmentation of Cutaneous Immune Responses by ATPγS: Purinergic Agonists Define a Novel Class of Immunologic Adjuvants
| Autor: | Anna Di Nardo, Jing Huang, Wanhong Ding, Richard D. Granstein, Richard L. Gallo, John A. Wagner, Aton M. Holzer |
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| Rok vydání: | 2005 |
| Předmět: |
Purinergic P2 Receptor Agonists
Agonist Mice Inbred A medicine.drug_class T-Lymphocytes medicine.medical_treatment Immunology Pharmacology Biology Dermatitis Contact Cell Line Purinergic Agonists Mice Adenosine Triphosphate Immune system Adjuvants Immunologic Antigen Antigens CD Antigens Neoplasm medicine Animals Immunology and Allergy RNA Messenger Receptor Antigen Presentation Mice Inbred BALB C Mice Inbred C3H Membrane Glycoproteins Receptors Purinergic P2 Histocompatibility Antigens Class II Interleukin-12 Clone Cells Interleukin-10 Cell culture Langerhans Cells B7-1 Antigen B7-2 Antigen Epidermis Adjuvant CD80 Interleukin-1 |
| Zdroj: | The Journal of Immunology. 174:7725-7731 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.174.12.7725 |
| Popis: | Extracellular nucleotides activate ligand-gated P2XR ion channels and G protein-coupled P2YRs. In this study we report that intradermal administration of ATPγS, a hydrolysis-resistant P2 agonist, results in an enhanced contact hypersensitivity response in mice. Furthermore, ATPγS enhanced the induction of delayed-type hypersensitivity to a model tumor vaccine in mice and enhanced the Ag-presenting function of Langerhans cells (LCs) in vitro. Exposure of a LC-like cell line to ATPγS in the presence of LPS and GM-CSF augmented the induction of I-A, CD80, CD86, IL-1β, and IL-12 p40 while inhibiting the expression of IL-10, suggesting that the immunostimulatory activities of purinergic agonists in the skin are mediated at least in part by P2Rs on APCs. In this regard, an LC-like cell line was found to express mRNA for P2X1, P2X7, P2Y1, P2Y2, P2Y4, P2Y9, and P2Y11 receptors. We suggest that ATP, when released after trauma or infection, may act as an endogenous adjuvant to enhance the immune response, and that P2 agonists may augment the efficacy of vaccines. |
| Databáze: | OpenAIRE |
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