Geranylgeraniol Causes a Decrease in Levels of Calreticulin and Tyrosine Phosphorylation of a 36-kDa Protein Prior to the Appearance of Apoptotic Features in HL-60 Cells
| Autor: | Shigemitsu Ohsawa, Yutaka Masuda, Mitsuru Okamoto, Shigeo Nakajo, I. Sakai, Kazuyasu Nakaya |
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| Rok vydání: | 1996 |
| Předmět: |
Time Factors
Transcription Genetic Biophysics Apoptosis HL-60 Cells Tumor cells Protein tyrosine phosphatase Biochemistry chemistry.chemical_compound Geranylgeraniol Humans Amino Acid Sequence RNA Messenger Phosphorylation Phosphotyrosine Molecular Biology biology Calcium-Binding Proteins Tyrosine phosphorylation Cell Biology Phosphoproteins Peptide Fragments Cell biology Molecular Weight Kinetics Ribonucleoproteins chemistry biology.protein Diterpenes Calreticulin Molecular Chaperones |
| Zdroj: | Biochemical and Biophysical Research Communications. 226:741-745 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1996.1423 |
| Popis: | It was demonstrated recently that geranylgeraniol (GGO) has potent apoptosis-inducing activity in various lines of tumor cells, including HL-60 cells. In the present study, we found that GGO markedly inhibited the expression of a calcium-binding protein, calreticulin, prior to the induction of apoptosis in HL-60 cells. Furthermore, we also observed a significant decrease in the tyrosine phosphorylation of a 36-kDa protein that is a major tyrosine-phosphorylated protein in HL-60 cells. These findings suggest that decreases in levels of calreticulin and in the tyrosine phosphorylation of the 36-kDa protein might be associated with the induction of apoptosis by GGO in HL-60 cells. |
| Databáze: | OpenAIRE |
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