Interleukin-10 Versus Dexamethasone: Effects on Polymorphonuclear Leukocyte Functions of the Newborn

Autor: Veronika Miskolci, Ivana Vancurova, Dennis Davidson, Brett V Citarella
Rok vydání: 2009
Předmět:
Cytotoxicity
Immunologic
Lipopolysaccharides
medicine.medical_specialty
Time Factors
Neutrophils
medicine.drug_class
medicine.medical_treatment
Anti-Inflammatory Agents
Apoptosis
Granulocyte
Dexamethasone
Article
chemistry.chemical_compound
Phagocytosis
Internal medicine
polycyclic compounds
medicine
Humans
Cells
Cultured
Respiratory Burst
Dose-Response Relationship
Drug
Caspase 3
business.industry
Infant
Newborn
N-Formylmethionine leucyl-phenylalanine
Fetal Blood
Receptor antagonist
Interleukin-10
Respiratory burst
N-Formylmethionine Leucyl-Phenylalanine
Chemotaxis
Leukocyte
Interleukin 10
Cytokine
Endocrinology
medicine.anatomical_structure
Neutrophil Infiltration
chemistry
Pediatrics
Perinatology and Child Health
Cytokines
Tumor necrosis factor alpha
business
hormones
hormone substitutes
and hormone antagonists
medicine.drug
Zdroj: Pediatric Research. 65:425-429
ISSN: 1530-0447
0031-3998
DOI: 10.1203/pdr.0b013e318199384d
Popis: Interleukin-10 (IL-10), an anti-inflammatory cytokine, may have therapeutic potential in the fetal inflammatory response syndrome and its sequelae such as bronchopulmonary dysplasia (BPD). Our aim was to compare the effects of IL-10 versus dexamethasone (DEX) on important PMN functions of the newborn. PMNs were isolated into culture medium from cord blood after elective cesarean section deliveries. IL-10 and DEX were compared on an equimolar basis corresponding to previously measured plasma levels of DEX from infants treated for BPD. The endotoxin (LPS)-stimulated release of the pro-inflammatory cytokines, tumor necrosis factor (TNFalpha) and IL-1 beta, were markedly inhibited equally by IL-10 and DEX; the anti-inflammatory cytokine IL-4 was not released and IL-1 receptor antagonist (IL-1ra) was released less with DEX compared with IL-10. PMNs exposed to LPS, N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), or S. aureus did not show a significant difference between control, DEX and IL-10 for apoptosis, respiratory burst, phagocytosis or killing respectively. Chemotaxis to fMLP or IL-8 was unaffected by DEX or IL-10. The principal effects of both IL-10 and DEX, on the PMN functions studied, are related to the control of pro- and anti-inflammatory cytokine release.
Databáze: OpenAIRE
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