Natural infection of cortelezzii complex (Diptera: Psychodidae: Phlebotominae) with Leishmania braziliensis in Chaco, Argentina
| Autor: | Reginaldo Peçanha Brazil, José Dilermando Andrade Filho, Juan R. Rosa, Oscar Daniel Salomón, Enrique Alejandro Szelag, Daniela de Pita Pereira |
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| Rok vydání: | 2012 |
| Předmět: |
Male
MULTIPLEX PCR ASSAY Phlebotominae Otras Ciencias Biológicas Veterinary (miscellaneous) Immunoblotting Leishmaniasis Cutaneous Zoology Polymerase Chain Reaction Leishmania braziliensis Ciencias Biológicas DRY CHACO ACL VECTOR parasitic diseases Animals Humans DOT BLOT ANALISYS Psychodidae Nyssomyia neivai biology Ecology Outbreak Species diversity biology.organism_classification Leishmania Insect Vectors Infectious Diseases Insect Science Vector (epidemiology) Female Parasitology HYBRIDIZATION CIENCIAS NATURALES Y EXACTAS Polymorphism Restriction Fragment Length |
| Zdroj: | Acta Tropica. 123:128-131 |
| ISSN: | 0001-706X |
| DOI: | 10.1016/j.actatropica.2012.04.008 |
| Popis: | In Argentina, American Cutaneous Leishmaniasis (ACL) extends up to 29°S in the phytogeographic regions of the Yungas (west), Chaco (center) and Paranaense (east). Since the Phlebotominae vectors of this disease in the western Chaco (dry Chaco) are unknown, in the present work, we studied the natural infection in Phlebotominae by PCR-ERFLP and Dot blot in order to incriminate these organisms as potential vectors. Captures with CDC-type traps were performed monthly in the domicile, the peridomicile and the forest in the Municipio Misión Nueva Pompeya, Chaco, Argentina, in two sites with human cases of ACL: Los Pozos (24°54′S, 61°22′W) and Fortín Arenales (24°58′S, 61°21′W), from November 2006 to December 2007. A total of 1702 Phlebotominae were captured: Mygonemyia migonei (83.8%), cortelezzii complex (11.1%), Mycropigomyia peresi (3.3%), Mycropygomy quinquefer (1.2%), Pintomyia torresi (0.2%) and Nyssomyia neivai (0.2%). Although no significant differences were found in species diversity, there were significant differences in abundance between both sites studied. A total of 80 phlebotomine females were analyzed: 50 of the cortelezzii complex and 30 My. migonei. No intestinal flagellates were observed by light microscopy. Two pools of 10 individuals of the cortelezzii complex of the peridomicile and forest of Fortín Arenales were reactive by PCR and Dot blot for Leishmania (Viannia) braziliensis. In Argentina, Evandromyia cortelezzii has been incriminated as a likely vector of ACL because of its abundance in areas of sporadic outbreaks. In the present work, Ev. cortelezzii females were found naturally infected, thus reinforcing the hypothesis that the members of the cortelezzii complex act as vectors of the disease. Fil: Rosa, Juan Ramón. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina Fil: Pita Pereira, Daniela. Instituto Oswaldo Cruz; Brasil Fil: Peçanha Brazil, Reginaldo. Instituto Oswaldo Cruz; Brasil Fil: Andrade Filho, José Dilermando. Instituto Oswaldo Cruz; Brasil Fil: Salomón, Oscar Daniel. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Szelag, Enrique Alejandro. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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