Effects of the calcium ionophore A23187 on airway responsiveness to histamine and substance P in guinea pigs
| Autor: | Hideo Tsukagoshi, Daisuke Uno, Masatomo Mori, Shigenori Iwamae, Takeshi Hisada |
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| Rok vydání: | 1997 |
| Předmět: |
Male
medicine.medical_specialty Immunology Population Guinea Pigs Drug Evaluation Preclinical Vascular permeability Bronchi Substance P Bronchial Provocation Tests chemistry.chemical_compound Internal medicine medicine Animals education Calcimycin Evans Blue Pharmacology education.field_of_study medicine.diagnostic_test Ionophores Microcirculation respiratory system Extravasation respiratory tract diseases Trachea Bronchoalveolar lavage Endocrinology chemistry Respiratory epithelium Airway Bronchoalveolar Lavage Fluid Histamine |
| Zdroj: | Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 46(3) |
| ISSN: | 1023-3830 |
| Popis: | Objective: We evaluated the mechanism of the airway hyperresponsiveness (AHR) induced by a calcium ionophore in guinea pigs. ¶Materials and Methods: Airway responsiveness to intravenous histamine (HS) and substance P (SP) was measured 24 h after a 1-h exposure to aerosolized A23187 (0.03 or 0.1 mg/ml) or its vehicle (10% DMSO). Changes were assessed by calculating – logPC350HS and – logPC350SP. Neutral endopeptidase (NEP) activity in the airway tissues, as well as the nitrite (NO2 −) levels and the cell population in bronchoalveolar lavage fluid (BALF) was determined after measurement of pulmonary function. Changes in SP-induced vascular permeability 24 h after exposure to A23187 were measured by the Evans Blue dye extravasation technique. ¶Results: Exposure to A23187 caused a significant AHR to SP, along with a significant increase in the number of neutrophils and epithelial cells in the BALF. While there was no significant change in NEP activity in the airway tissues, the levels of nitrite in the BALF were significantly decreased in A23187-exposed animals. Significant correlations were found between the number of epithelial cells in the BALF and – logPC350SP (r = 0.477, p |
| Databáze: | OpenAIRE |
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