Choline attenuates inflammatory hyperalgesia activating nitric oxide/cGMP/ATP-sensitive potassium channels pathway
| Autor: | Ricardo Kusuda, Eleonora U Carreira, Luis Ulloa, Alexandre Kanashiro, Thiago M. Cunha, Fernando Q. Cunha |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Agonist Male NOCICEPTIVIDADE alpha7 Nicotinic Acetylcholine Receptor medicine.drug_class Freund's Adjuvant Inflammation Pharmacology Nitric Oxide Dinoprostone Article Nitric oxide Choline 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine KATP Channels medicine Animals Molecular Biology Cyclic GMP Mice Inbred BALB C biology General Neuroscience Potassium channel Nitric oxide synthase 030104 developmental biology Nociception chemistry Hyperalgesia biology.protein Neurology (clinical) medicine.symptom 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | New findings on neural regulation of immunity are allowing the design of novel pharmacological strategies to control inflammation and nociception. Herein, we report that choline, a 7-nicotinic acetylcholine receptor (α7nAChRs) agonist, prevents carrageenan-induced hyperalgesia without affecting inflammatory parameters (neutrophil migration or cytokine/chemokines production) or inducing sedation or even motor impairment. Choline also attenuates prostaglandin-E(2) (PGE(2))-induced hyperalgesia via α7nAChR activation and this anti-nociceptive effect was abrogated by administration of LNMMA (a nitric oxide synthase inhibitor), ODQ (an inhibitor of soluble guanylate cyclase; cGMP), and glibenclamide (an inhibitor of ATP-sensitive potassium channels). Furthermore, choline attenuates long-lasting Complete Freund’s Adjuvant and incision-induced hyperalgesia suggesting its therapeutic potential to treat pain in rheumatoid arthritis or post-operative recovery, respectively. Our results suggest that choline modulates inflammatory hyperalgesia by activating the nitric oxide/cGMP/ATP-sensitive potassium channels without interfering in inflammatory events, and could be used in persistent pain conditions. |
| Databáze: | OpenAIRE |
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