Cytochemical studies on Endamoeba histolytica, with particular reference to polysaccharides
| Autor: | Joseph B. Michaelson, James N. DeLamater, Frances A. Hallman, Herbert Blumenthal |
|---|---|
| Rok vydání: | 1955 |
| Předmět: |
Saliva
Starch Immunology Polysaccharide Microbiology chemistry.chemical_compound Endamoeba Polysaccharides parasitic diseases Coloring Agents chemistry.chemical_classification Staining and Labeling biology Entamoeba histolytica Mucin Metachromasia General Medicine biology.organism_classification Staining Infectious Diseases Biochemistry chemistry biology.protein Carbohydrate Metabolism Parasitology Alpha-amylase |
| Zdroj: | Experimental Parasitology. 4:45-53 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/0014-4894(55)90022-9 |
| Popis: | Cytochemical staining reactions indicate that cultured amebae and amebae from tissue contain polysaccharides. The effect of prior exposure to beta-glucuronidase and to saliva on the staining reaction of amebae from cultures containing starch suggests that more than one type of polysaccharide may exist in these cells. The lack of activity of these enzymatic substances on the staining reaction of amebae from starch-free cultures and from tissues would indicate that the polysaccharide content of amebae varies under different conditions of nutrition. When stained by Toluidine blue O, metachromasia is slight or absent in amebae from starch cultures and in those from liver tissue, but is marked in amebae from starch-free cultures. |
| Databáze: | OpenAIRE |
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