Detection of human cytomegalovirus in synovial neutrophils obtained from patients with rheumatoid arthritis
| Autor: | Cecilia Söderberg-Nauclér, Xinling Xu, Belghis Davoudi, Afsar Rahbar, Vivianne Malmström, Stefania Varani, A Estekizadeh |
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| Přispěvatelé: | Xu X., Estekizadeh A., Davoudi B., Varani S., Malmstrom V., Rahbar A., Soderberg-Naucler C. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
musculoskeletal diseases
Human cytomegalovirus Male cytomegalovirus rheumatoid arthritis polymorphonuclear leucocytes Neutrophils viruses Immunology Cytomegalovirus Arthritis Rheumatoid Viral Matrix Proteins 03 medical and health sciences 0302 clinical medicine Rheumatology medicine Immunology and Allergy Humans 030212 general & internal medicine 030203 arthritis & rheumatology business.industry Synovial Membrane virus diseases General Medicine medicine.disease Rheumatoid arthritis Immunoglobulin G Female business |
| Popis: | Objectives: To examine whether signs of an active human cytomegalovirus (HCMV) infection are present in affected joints of patients with rheumatoid arthritis (RA). Method: Polymorphonuclear leucocytes (PMNLs) were obtained from synovial fluid (SF) of 17 RA patients and were analysed for HCMV-pp65 and HCMV-immediate early (IE) proteins using the antigenemia assay. Peripheral blood (PB) and SF obtained from these 17 patients and from 17 additional RA patients (n = 34) were tested for HCMV-IE and pp150 DNA with Taqman polymerase chain reaction. Plasma samples from the patients were analysed for HCMV-immunoglobulin M (IgM) and immunoglobulin G (IgG) by enzyme-linked immunosorbent assay and compared to 71 healthy gender-matched blood donors. Results: HCMV-pp65 protein was detected in 65% of synovial PMNL samples, but in only 18% of PMNLs from PB. In contrast, HCMV IE protein was not found in any of the analysed PMNL samples. On the DNA level, HCMV-IE and pp150 DNA was detected in SF of 13/32 (41%) and 14/23 (61%) of RA patients, respectively. HCMV-IE and pp150 DNA was also found in 24/33 (73%) and in 16/24 (67%) of PB samples obtained from RA patients, respectively. HCMV IgG seroprevalence was 76% in RA patients as well as in healthy controls, while only one RA patient was positive for specific IgM. Conclusions: HCMV pp65 antigen was found in PMNLs from SF of RA patients, indicating an active infection in the affected joint. Future studies are needed to determine whether HCMV infection can aggravate the inflammatory process in these patients. |
| Databáze: | OpenAIRE |
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