Proximity of the Start Codon to a Leaderless mRNA's 5′ Terminus Is a Strong Positive Determinant of Ribosome Binding and Expression in Escherichia coli
| Autor: | Gary R. Janssen, William J. Van Etten, Karthik Krishnan |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Ribosomal Proteins
Genetics Messenger RNA Codon Initiator RNA Shine-Dalgarno sequence Gene Expression Regulation Bacterial Biology Microbiology Ribosome Ribosomal binding site Start codon Ribosomal protein Escherichia coli Coding region Gene Regulation RNA Messenger 5' Untranslated Regions Ribosomes Molecular Biology Protein Binding |
| Zdroj: | Journal of Bacteriology. 192:6482-6485 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | An AUG start codon is an important determinant of ribosome binding and expression of leaderless mRNAs in Escherichia coli . Using reporter constructs encoding mRNAs where the AUG start codon is preceded by untranslated leaders of various length and sequence, we find that close proximity of the start codon to the 5′ terminus and the leader sequence are strong determinants of both ribosome binding and expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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