Hair Follicle–Derived Blood Vessels Vascularize Tumors in Skin and Are Inhibited by Doxorubicin
| Autor: | Robert M. Hoffman, Yasuyuki Amoh, Ping Jiang, Meng Yang, Lingna Li, Abdool R. Moossa, Kensei Katsuoka |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
CD31
Cancer Research Pathology medicine.medical_specialty Skin Neoplasms Angiogenesis Recombinant Fusion Proteins Green Fluorescent Proteins Melanoma Experimental Mice Transgenic Nerve Tissue Proteins Cell Growth Processes Biology Nestin Neovascularization Mice Intermediate Filament Proteins medicine Animals Neovascularization Pathologic Melanoma medicine.disease Hair follicle Transplantation medicine.anatomical_structure Oncology Doxorubicin medicine.symptom Hair Follicle Blood vessel |
| Zdroj: | Cancer Research. 65:2337-2343 |
| ISSN: | 1538-7445 0008-5472 |
| DOI: | 10.1158/0008-5472.can-04-3857 |
| Popis: | We have recently shown that the neural-stem cell marker nestin is expressed in hair follicle stem cells and the blood vessel network interconnecting hair follicles in the skin of transgenic mice with nestin regulatory element–driven green fluorescent protein (ND-GFP). The hair follicles were shown to give rise to the nestin-expressing blood vessels in the skin. In the present study, we visualized tumor angiogenesis by dual-color fluorescence imaging in ND-GFP transgenic mice after transplantation of the murine melanoma cell line B16F10 expressing red fluorescent protein. ND-GFP was highly expressed in proliferating endothelial cells and nascent blood vessels in the growing tumor. Results of immunohistochemical staining showed that the blood vessel–specific antigen CD31 was expressed in ND-GFP–expressing nascent blood vessels. ND-GFP expression was diminished in the vessels with increased blood flow. Progressive angiogenesis during tumor growth was readily visualized during tumor growth by GFP expression. Doxorubicin inhibited the nascent tumor angiogenesis as well as tumor growth in the ND-GFP mice transplanted with B16F10-RFP. This model is useful for direct visualization of tumor angiogenesis and evaluation of angiogenic inhibitors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|