T cell factor-activated transcription is not sufficient to induce anchorage-independent growth of epithelial cells expressing mutant β-catenin
| Autor: | Angela I.M. Barth, W J Nelson, D. B. Stewart |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Transcriptional Activation
Beta-catenin Cell division T cell Molecular Sequence Data Mutant Biology Dogs Transcription (biology) Tumor Cells Cultured medicine Animals Humans Transcription factor beta Catenin Multidisciplinary Base Sequence Cell growth Epithelial Cells Biological Sciences Molecular biology Cell biology Cytoskeletal Proteins Cell Transformation Neoplastic medicine.anatomical_structure Mutation Trans-Activators biology.protein Signal transduction Cell Division Signal Transduction Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences. 96:4947-4952 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.96.9.4947 |
| Popis: | N-terminal mutations in beta-catenin that inhibit beta-catenin degradation are found in primary tumors and cancer cell lines, and increased beta-catenin/T cell factor (TCF)-activated transcription in these cells has been correlated with cancer formation. However, the role of mutant beta-catenin in cell transformation is poorly understood. Here, we compare the ability of different N-terminal mutations of beta-catenin (DeltaN131, DeltaN90, DeltaGSK) to induce TCF-activated transcription and anchorage-independent growth in Madin-Darby canine kidney epithelial cells. Expression of DeltaN90 or DeltaGSK beta-catenin increased TCF-activated transcription but did not induce significant anchorage-independent cell growth. In contrast, deletion of the alpha-catenin-binding site in DeltaN131 beta-catenin reduced TCF-activated transcription, compared with that induced by DeltaN90 or DeltaGSK beta-catenin, but significantly enhanced anchorage-independent cell growth. |
| Databáze: | OpenAIRE |
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