Antibiograms and randomly amplified polymorphic DNA-polymerase chain reactions (RAPD-PCR) as epidemiological markers of gonorrhea
| Autor: | Ratana Lawung, Leif Bülow, Virapong Prachayasittikul, Rungrot Cherdtrakulkiat, Angkana Charoenwatanachokchai, Tharinda Mungniponpan, Sivarak Thammapiwan |
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| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Spectinomycin Clinical Biochemistry Drug resistance Microbial Sensitivity Tests Biology medicine.disease_cause Benzylpenicillin Polymerase Chain Reaction law.invention Microbiology Gonorrhea Antibiotic resistance Bacterial Proteins law Drug Resistance Bacterial medicine Immunology and Allergy Humans Polymerase chain reaction Molecular Epidemiology Chi-Square Distribution Biochemistry (medical) Public Health Environmental and Occupational Health Hematology Original Articles bacterial infections and mycoses Thailand Virology Neisseria gonorrhoeae RAPD Anti-Bacterial Agents Random Amplified Polymorphic DNA Technique Penicillin Medical Laboratory Technology DNA Gyrase medicine.drug |
| Zdroj: | Journal of clinical laboratory analysis. 24(1) |
| ISSN: | 1098-2825 |
| Popis: | The development of antimicrobial resistance of Neisseria gonorrhoeae arising from wide dissemination of resistant clones is a major global health problem. In this study, a total of 235 isolates of N. gonorrhoeae isolated from patients of Bangrak Hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, ofloxacin, ciprofloxacin, spectinomycin, and ceftriaxone. Mutation (Ser‐91) in the quinolone resistance determining regions of gyrA and random amplification of the polymorphic DNA polymerase chain reaction (RAPD‐PCR) were examined from 145 isolates. Among these, 55 isolates were obtained during January–March 2000, 46 isolates during January–March 2002, and 44 isolates during October–December 2002. The occurrence of combination resistance between penicillin and quinolone was 20% in January–March 2000, which was increased to 57.8% during the period of October–December 2002 (P |
| Databáze: | OpenAIRE |
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