Development of Time-Resolved Fluorescence Immunochromatographic Assays for Simultaneously Detecting Tylosin and Tilmicosin in Milk in Group-Screening Manner
| Autor: | Ying He, Hong Wang, Jian-Xin Huang, Yu-Dong Shen, Yu Wang, Zhen-Lin Xu, Lu Li, Yuanxin Tian, Jin-Yi Yang |
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| Rok vydání: | 2021 |
| Předmět: |
Detection limit
milk tylosin Health (social science) Chromatography Group screening Chemical technology TP1-1185 Plant Science time-resolved fluorescence immunochromatographic assay Tylosin tilmicosin Health Professions (miscellaneous) Microbiology Article Milk sample Standard curve chemistry.chemical_compound Immunoassay method chemistry Immunochromatographic Assays Tilmicosin Food Science |
| Zdroj: | Foods Foods, Vol 10, Iss 1838, p 1838 (2021) Volume 10 Issue 8 |
| ISSN: | 2304-8158 |
| DOI: | 10.3390/foods10081838 |
| Popis: | Tylosin and tilmicosin (T& T) residues in livestock products have received extensive attention from consumers. Time-resolved fluorescence immunochromatographic assay (TRFICA), as a fast, efficient and sensitive immunoassay method, has played an increasingly important role in the food safety field. Therefore, herein a quantitative and visual TRFICA was established for simultaneously detecting T& T in milk in a group-screening manner. Under the optimal conditions, the standard curve range of developed TRFICA based on the T& T was 1.87~7.47 ng/mL, and the half-maximal inhibition concentrations (IC50) were 4.06 ng/mL and 3.74 ng/mL, respectively. The limits of detection (LOD) of the TRFICA method were from 1.72 ng/mL to 1.39 ng/mL, and the visual cut-off values were 31.25 ng/mL and 62.50 ng/mL for T& T in milk, respectively. Moreover, the stability experiments showed that the strips could be stored at 4 °C for more than 6 months, the total detection time was less than 13 min, and the cross-reactivities (CRs) with related compounds were less than 0.1%, which concluded that the developed TRFICA method could be used in real milk sample detection. |
| Databáze: | OpenAIRE |
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