3D Structure of the human genome: order in randomness
| Autor: | Eva Bártová, Alena Ganová, Martin Falk, Pavla Jirsová, Emilie Lukášová, Stanislav Kozubek, Irena Koutná, Renata Paseková, Michal Kozubek |
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| Rok vydání: | 2002 |
| Předmět: |
Genetics
Cell Nucleus Genome Human Chromosome Gene Expression Computational biology DNA Biology Chromatin Cell nucleus medicine.anatomical_structure Imaging Three-Dimensional Centromere medicine Homologous chromosome Humans Nucleic Acid Conformation Human genome Scaffold/matrix attachment region Gene Genetics (clinical) In Situ Hybridization Fluorescence |
| Zdroj: | Chromosoma. 111(5) |
| ISSN: | 0009-5915 |
| Popis: | A complex study of the spatial arrangement of different genetic elements (genes, centromeres and chromosomal domains) in the cell nucleus is presented and the principles of this arrangement are discussed. We show that the radial location of genetic elements in the three-dimensional (3D) space between the center of the nucleus and the nuclear membrane is element specific and dependent on the position of the element on the chromosome. In contrast, mutual angular positioning of both homologous and heterologous genetic elements is, in the majority of cases, random. In several cases, tethering of heterologous genetic elements was observed. This close proximity of specific loci may be responsible for their mutual rearrangement and the development of cancer. Comparison of our results with transcriptome maps shows that the nuclear location of chromosomal domains with highly expressed genes is more central when compared with chromosomes with low expression. The higher-order chromatin structure is strikingly similar in various human cell types, which correlates with the fact that the profiles of gene expression are also similar. |
| Databáze: | OpenAIRE |
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