The role of intestinal endotoxemia in a rat model of aluminum neurotoxicity

Autor: Feng Wang, Wen‑Xing Li, Li‑Xin Liu, Rui‑Xia Guo, De‑Wu Han, Bai Han, Bao‑Feng Yu
Rok vydání: 2017
Předmět:
Lipopolysaccharides
Male
0301 basic medicine
Cancer Research
Pathology
Lipopolysaccharide
D-galactose
Morris water navigation task
Biochemistry
chemistry.chemical_compound
0302 clinical medicine
aluminum neurotoxicity
Aluminum Chloride
Aspartic Acid Endopeptidases
Intestinal Mucosa
Aluminum Compounds
Glutaminase
lipopolysaccharide
Interleukin
Articles
Intestines
Oncology
Blood-Brain Barrier
Molecular Medicine
Tumor necrosis factor alpha
Diamine oxidase
medicine.medical_specialty
Neurotoxins
Biology
03 medical and health sciences
aluminum trichloride
Chlorides
Memory
Internal medicine
Presenilin-1
Genetics
medicine
Animals
RNA
Messenger
Rats
Wistar
Molecular Biology
intestinal endotoxemia
amyloid β-protein
Amyloid beta-Peptides
Tumor Necrosis Factor-alpha
Neurotoxicity
Galactose
medicine.disease
Endotoxemia
Peptide Fragments
Glutamine
Disease Models
Animal
030104 developmental biology
Endocrinology
chemistry
Zonula Occludens-1 Protein
Muramidase
Amyloid Precursor Protein Secretases
030217 neurology & neurosurgery
Aluminum
Interleukin-1
Zdroj: Molecular Medicine Reports
ISSN: 1791-3004
1791-2997
Popis: The present study aimed to investigate the effects of intestinal endotoxemia (IETM) in a rat model of aluminum neurotoxicity established by D-galactose and aluminum trichloride (AlCl3). Adult Wistar rats were administered D‑galactose and AlCl3 to create the aluminum neurotoxicity model. The learning and memory abilities of the rats were subsequently observed using a Morris water maze test and the serum levels of lipopolysaccharide (LPS), tumor necrosis factor (TNF)‑α, interleukin (IL)‑1, diamine oxidase (DAO), glutamine (Gln) and glutaminase were measured. The expression of S‑100β in the serum was detected using an enzyme‑linked immunosorbent assay. The expression levels of the amyloid β‑protein (Aβ) precursor (APP), presenilin 1 (PS1), β‑site APP‑cleaving enzyme (BACE), zona occludens protein (ZO)‑1 and Aβ 1‑40 in the brain of rats were detected via reverse‑transcription polymerase chain reaction, western blotting and immunohistochemistry. The levels of LPS, TNF‑α, IL‑1, DAO, Gln and S‑100β in serum and the mRNA and protein expression levels of APP, PS1, BACE and Aβ1‑40 in the brain were markedly increased in the model rats compared with controls. The level of glutaminase in the serum and the expression of ZO‑1 in the brain were decreased in the model rats compared with controls. IETM was present in the rat model of aluminum neurotoxicity established by D‑galactose and AlCl3 and may be important in the development of this neurotoxicity.
Databáze: OpenAIRE
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